Langmuir-Blodgett monolayer films of the Rhodopseudomonas viridis reaction center: determination of the order of the hemes in the cytochrome c subunit.
Langmuir-Blodgett monolayer films of the Rhodopseudomonas viridis reaction center: determination of the order of the hemes in the cytochrome c subunit.
复制标题
绿色红假单胞菌反应中心的 Langmuir-Blodgett 单层膜：细胞色素 c 亚基中血红素顺序的测定。
DOI：
10.1016/s0005-2728(05)80108-2
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Dutton,PL
中科院分区：
文献类型：
--
作者：
Alegria,G;Dutton,PL
利用Langmuir-Blodgett(LB)膜技术，从绿色红假单胞菌的光合作用细菌分离的反应中心细胞色素复合体(RC-Cytc)和发色团膜中制备了定向和光活性薄膜。用线性二色性(LD)和氧化还原电位法对RC-Cytc络合物的四种细胞色素进行了鉴定。在分离的RC-Cyt-C络合物和发色团膜的LB膜中，四种血红素的可分辨的角取向允许将24种可能的排列减少到两种。来自其他来源的额外结构和功能信息使我们能够提出一个最好地解释所有可用实验数据的模型。
The Langmuir-Blodgett (LB) film technique has been applied to produce oriented and photo-active films of isolated reaction center cytochromeccomplexes (RC-cytc) and chromatophore membranes from the photosynthetic bacteriumRhodopseudomonas viridis. Linear dichroism (LD) and redox potentiometry have been used to identify the four cytochromechemes of the RC-cytccomplex. Resolved angular orientations of the four hemes in LB films of both isolated RC-cyt-ccomplexes and of chromatophore membranes permit the reduction of the 24 possible arrangements to two. Additional structural and functional information from other sources allows us to propose a model which best accounts for all the available experimental data.