Mechanical properties of the collagen network in human articular cartilage as measured by osmotic stress technique
Mechanical properties of the collagen network in human articular cartilage as measured by osmotic stress technique
复制标题
DOI：
10.1006/abbi.1997.0507
复制
发表时间：
1998-03-15
影响因子：
3.9
通讯作者：
Maroudas, A
中科院分区：
文献类型：
--
作者：
Basser, PJ;Schneiderman, R;Maroudas, A
我们使用各向同性渗透应力技术来评估人体关节软骨在各种水合情况下的膨胀压力，以便首次根据这些测量结果确定胶原蛋白网络中的张应力 P-c 作为水合的函数。通过校准的聚乙二醇溶液施加渗透应力。渗透应力的计算基于作用于原纤维外基质隔室内的施加应力、胶原应力和蛋白聚糖渗透压pi(PG)之间的平衡。对几个正常人体样本（天然样本和胰蛋白酶处理样本）以及来自一个骨关节炎 (OA) 关节的软骨测定了 P-c 与水合的关系。我们发现对于正常软骨来说，胶原蛋白网络。直到软骨体积减少其初始值的 20-25% 时，它才会变得“软弱”，因此必须在比之前想象的更广泛的水合作用范围内考虑其对软骨力平衡的贡献。对于正常软骨，P-c 与水化曲线随着水化程度的增加而急剧增加；胰蛋白酶处理不会改变其斜率，表明 PC；浓度不影响胶原蛋白网络的固有刚度。相比之下，OA 样本的曲线相当浅，并转移到更高的水合作用，因此我们的研究结果强调了胶原蛋白网络的刚度在限制正常软骨中的水合作用并确保基质中高 PG 浓度方面的作用，这对于有效承载至关重要，但在 OA 中会丢失。 (C) 1998 年学术出版社。
We have used an isotropic osmotic stress technique to assess the swelling pressures of human articular cartilage over a wide range of hydrations in order to determine from these measurements, for the first time, the tensile stress in the collagen network, P-c, as a function of hydration. Osmotic stress was applied by means of calibrated solutions of polyethylene glycol, Calculations of osmotic stress were based on the balance, at equilibrium, between the applied stress, the collagen stress, and the proteoglycan osmotic pressure, pi(PG), acting within the extrafibrillar matrix compartment. P-c vs hydration was determined for several normal human samples, both native and trypsin-treated, and for cartilage from one osteoarthritic (OA) joint. We found that for normal cartilage the collagen network. does not become "limp" until the volume of cartilage has decreased by 20-25% of its initial value and that its contribution to the balance of forces in cartilage therefore must be taken into account over a much wider range of hydrations than was previously thought. For normal cartilage, the P-c vs hydration curves exhibit a steep increase with increasing hydration; trypsin treatment does not change their slope, showing that PC; concentration does not influence the inherent stiffness of the collagen network. By contrast, the curves for OA specimens are considerably shallower and displaced to higher hydrations, Our findings thus highlight the role of the stiffness of the collagen network in limiting hydration in normal cartilage and ensuring a high PG concentration in the matrix, which is essential for effective load-bearing and is lost in OA. (C) 1998 Academic Press.