Sustained Inflammation Induces Degeneration of the Temporomandibular Joint
Sustained Inflammation Induces Degeneration of the Temporomandibular Joint
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DOI：
10.1177/0022034512441946
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发表时间：
2012-05-01
影响因子：
7.6
通讯作者：
Zhou, Y. H.
中科院分区：
文献类型：
--
作者：
Wang, X. D.;Kou, X. X.;Zhou, Y. H.
关节炎患者的颞下颌关节 (TMJ) 会发生退行性变化，但人们对 TMJ 骨关节炎和类风湿性关节炎的发病机制知之甚少。我们假设颞下颌关节持续炎症会引起结构异常，并相应地在双次注射完全弗氏佐剂 (CFA) 的新型模型中使用行为、形态、细胞和分子评估来表征椎间盘和滑膜。对七周大的雌性 Sprague-Dawley 大鼠进行两次 CFA 注射后 35 天，CFA 诱导的炎症组中的椎间盘表现出多种退行性变化，包括明显增厚、不透明和变形。 CFA 组的椎间盘还显示出显着更大的湿重和净重，以及升高的胶原蛋白、蛋白聚糖和总糖胺聚糖含量。 CFA诱导炎症组的滑膜显示出明显的单核细胞浸润和滑膜下脂肪组织的积累。椎间盘和滑膜的 iNOS 和 IL-1 β mRNA 表达均显着高于对照组（注射盐水）。这些发现与我们的假设一致，即持续的颞下颌关节炎症，即使在目前观察到的 35 天内，也可能是结构异常的诱发因素。对颞下颌关节炎症和退变的深入了解预计将提高我们对颞下颌关节关节炎发病机制的理解，并帮助设计临床相关的组织工程策略。
The temporomandibular joint (TMJ) undergoes degenerative changes among patients who suffer from arthritis, and yet the pathogenesis of TMJ osteoarthritis and rheumatoid arthritis is poorly understood. We hypothesized that sustained inflammation in the TMJ induces structural abnormalities, and accordingly characterized the disc and synovium in a novel model with double injections of complete Freund's adjuvant (CFA), using behavioral, morphological, cellular, and molecular assessments. Thirty-five days following double CFA injections in seven-week-old female Sprague-Dawley rats, the disc in the CFA-induced inflammation group demonstrated multiple degenerative changes, including marked thickening, opacity, and deformation. The discs in the CFA group further showed significantly greater wet and net weights, and elevated collagen, aggrecan, and total glycosaminoglycan contents. The synovium in the CFA-induced inflammation group showed marked infiltration of mononucleated cells and accumulated sub-synovial adipose tissue. Both the disc and synovium had significantly higher iNOS and IL-1 beta mRNA expression than controls (saline injections). These findings are consistent with our hypothesis that sustained TMJ inflammation, even within the presently observed 35 days, may be a predisposing factor for structural abnormalities. Insight into TMJ inflammation and degeneration is anticipated to improve our understanding of the pathogenesis of TMJ arthritis and help design clinically relevant strategies for tissue engineering.