Immobilization of Candida rugosa lipase on hydrophobic/strong cation-exchange functional silica particles for biocatalytic synthesis of phytosterol esters
Immobilization of Candida rugosa lipase on hydrophobic/strong cation-exchange functional silica particles for biocatalytic synthesis of phytosterol esters
复制标题
疏水/强阳离子交换功能性二氧化硅颗粒上固定念珠菌脂肪酶用于生物催化合成植物甾醇酯
DOI：
10.1016/j.biortech.2011.11.128
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发表时间：
2012-07-01
影响因子：
11.4
通讯作者：
Huang, Feng-Hong
中科院分区：
文献类型：
--
作者：
Zheng, Ming-Ming;Lu, Yong;Huang, Feng-Hong
在这项工作中，与辛基和磺酸基团功能化的混合模式的二氧化硅颗粒方便地通过共键合的正辛基三乙氧基硅烷和3-巯基丙基三乙氧基硅烷的混合物，然后用过氧化氢氧化制备。利用疏水性和强阳离子交换作用将皱褶假丝酵母脂肪酶（CRL）固定在混合型硅胶颗粒上。与游离CRL相比，固定化CRL在高温下的稳定性显著提高。将固定化CRL作为生物催化剂用于植物甾醇与游离脂肪酸（FFA）的酶促酯化反应，制备植物甾醇酯。在最佳工艺条件下，植物甾醇亚麻酸酯的酯化率可达95.3%。以混合型硅胶颗粒固定化CRL为生物催化剂，植物甾醇酯与脂肪酸甲酯（80.5%）或三酰甘油（95.5%以上）进行酯交换反应，可高产率地转化为相应的长链酰基酯。吸附法固定化的CRL经7次循环后仍保持其初始活性的78.6%。(C)2011爱思唯尔有限公司保留所有权利。
In this work, mixed-mode silica particles functionalized with octyl and sulfonic acid groups was conveniently prepared by co-bonding a mixture of n-octyltriethoxysilane and 3-mercaptopropyltriethoxysilane and then oxidized with hydrogen peroxide. Candida rugosa lipase (CRL) was immobilized on the mixed-mode silica particles via hydrophobic and strong cation-exchange interaction. The resulting immobilized CRL increased remarkably its stability at high temperature in comparison to free CRL. The immobilized CRL was used as biocatalysts for enzymatic esterification of phytosterols with free fatty acids (FFAs) to produce phytosterol esters. The phytosterols linolenate esterification degree of 95.3% was obtained under the optimized condition. Phytosterols esters could also been converted in high yields to the corresponding long-chain acyl esters via transesterification with methyl esters of fatty acids (80.5%) or triacylglycerols (above 95.5%) using mixed-mode silica particles immobilized CRL as biocatalyst. Furthermore, the immobilized CRL by absorption retained 78.6% of their initial activity after 7 recycles. (C) 2011 Elsevier Ltd. All rights reserved.