Manifestation of osteoblastic phenotypes in the sarcomatous component of epithelial carcinoma and sarcomatoid carcinoma
Manifestation of osteoblastic phenotypes in the sarcomatous component of epithelial carcinoma and sarcomatoid carcinoma
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DOI：
10.1177/1010428317704365
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发表时间：
2017-06-27
期刊：
影响因子：
--
通讯作者：
Ito, Akihiko
中科院分区：
文献类型：
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作者：
Takashima, Yasutoshi;Murakami, Teppei;Ito, Akihiko
上皮癌偶尔有肉瘤成分，主要由梭形和立方形细胞组成，通常类似于成骨细胞。肉瘤样癌由相似的细胞组成。最近的研究已经将这些现象表征为癌细胞中上皮-间充质转化的表现，但是在上皮癌的肉瘤细胞中表现的间充质表型尚不清楚。在这里，我们研究了四种成骨细胞分化生物标志物在多种癌类型的肉瘤成分中的表达谱，包括五种肾透明细胞癌、四种乳腺浸润性导管癌、两种食管癌、一种上颌鳞状细胞癌、三种喉癌、三种肺癌、一种肝癌和一种皮肤肉瘤样癌。使用抗细胞粘附分子1（IgCAM超家族成员）、osterix转录因子（Osterix）、分化簇151（跨膜4超家族成员）和碱性磷酸酶的抗体通过免疫组织化学分析表达。每种标记物的免疫染色强度以0级（阴性）、0.5级（弱）和1级（强）进行评级，并将四个量表值相加以计算成骨细胞评分。总共有10例患者的成骨细胞评分>= 3，并且这10例患者均为细胞粘附分子1和Osterix阳性。成骨细胞评分< 3的9个样本中，分别有8个和5个样本的细胞粘附分子1（p < 0.0001）和Osterix（p = 0.006）呈阴性。其他指标无统计学意义。这些结果表明，成骨细胞分化可以发生在癌细胞和细胞粘附分子1可能是一个有用的标志物，用于识别这种现象在癌组织。
Epithelial carcinomas occasionally have sarcomatous components that consist primarily of spindle and cuboidal cells, which often resemble osteoblasts. Sarcomatoid carcinomas consist of similar cells. Recent studies have characterized these phenomena as a manifestation of epithelial-mesenchymal transition in carcinoma cells, but the mesenchymal phenotypes that manifest in sarcomatous cells of epithelial carcinomas are not well understood. Here, we examined the expression profiles of four osteoblastic differentiation biomarkers in the sarcomatous components of multiple carcinoma types, including five renal clear cell, four breast invasive ductal, two esophageal, one maxillary squamous cell, three larynx, three lung, one liver, and one skin sarcomatoid carcinoma. Expression was analyzed by immunohistochemistry using antibodies against cell adhesion molecule 1, a member of the IgCAM superfamily, osterix transcription factor (Osterix), cluster of differentiation 151, a transmembrane 4 superfamily member, and alkaline phosphatase. Immunostaining intensity was rated in scale 0 (negative), 0.5 (weak), and 1 (strong) for each marker, and the four scale values were summed to calculate osteoblastic scores. In all, 10 cases had a osteoblastic score >= 3, and all of these 10 cases were cell adhesion molecule 1- and Osterix-positive. Eight and five of the nine samples with a osteoblastic score < 3 were negative for cell adhesion molecule 1 (p < 0.0001) and Osterix (p = 0.006), respectively. The other markers showed no statistical significance. These results indicate that osteoblastic differentiation can occur in carcinoma cells and that cell adhesion molecule 1 could be a useful marker for identifying this phenomenon in carcinoma tissues.