Simultaneous electrochemical detection of multiple tumor markers based on dual catalysis amplification of multi-functionalized onion-like mesoporous graphene sheets.
Simultaneous electrochemical detection of multiple tumor markers based on dual catalysis amplification of multi-functionalized onion-like mesoporous graphene sheets.
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DOI：
10.1016/j.aca.2012.08.018
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发表时间：
2012-10
影响因子：
6.2
通讯作者：
Jing Han;Ying Zhuo;Y. Chai;R. Yuan;W. Zhang;Qiang Zhu
中科院分区：
文献类型：
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作者：
Jing Han;Ying Zhuo;Y. Chai;R. Yuan;W. Zhang;Qiang Zhu
在这项工作中，制造了一种夹心型电化学免疫传感器，用于同时灵敏地检测前列腺特异性抗原（PSA）和游离前列腺特异性抗原（fPSA）。首先分别制备金纳米粒子（AuNPs）修饰的普鲁士蓝和六氰基铁酸镍纳米粒子，然后修饰洋葱状介孔石墨烯片（表示为Au@PBNPs/O-GS和Au@NiNPs/O-GS）作为可区分的信号标签来标记不同的检测抗体。随后，使用链霉亲和素和生物素化碱性磷酸酶（bio-AP）来封闭可能剩余的活性位点。利用所制备的纳米杂化物，在bio-AP存在下，抗坏血酸2-磷酸催化原位产生AA，然后AA分别被Au@PBNPs/O-GS和Au@NiNPs/O-GS纳米杂化物进一步催化，从而实现双催化放大，以获得更高的信号响应。实验结果表明，所提出的同时测定fPSA的免疫传感器的线性范围为0.02至10ngmL−1，检出限为6.7pgmL−1；PSA的线性范围为0.01至50ngmL−1，检出限为3.4pgmL−1（S/N=3）。重要的是，所提出的方法为生物样品的快速、简单和经济高效的分析提供了希望。
In this work, a sandwich-type electrochemical immunosensor for simultaneous sensitive detection of prostate specific antigen (PSA) and free prostate specific antigen (fPSA) is fabricated. Gold nanoparticles (AuNPs) modified Prussian blue and nickel hexacyanoferrates nanoparticles were firstly prepared, respectively, and then decorated onion-like mesoporous graphene sheets (denoted as Au@PBNPs/O-GS and Au@NiNPs/O-GS) as distinguishable signal tags to label different detection antibodies. Subsequently, streptavidin and biotinylated alkaline phosphatase (bio-AP) were employed to block the possible remaining active sites. With the employment of the as prepared nanohybrids, the dual catalysis amplification can be achieved by catalysis of the ascorbic acid 2-phosphate to in situ produce AA in the presence of bio-AP, and then AA was further catalyzed by Au@PBNPs/O-GS and Au@NiNPs/O-GS nanohybrids, respectively, to obtain the higher signal responses. The experiment results show that the linear range of the proposed immunosensor for simultaneous determination of fPSA is from 0.02 to 10ngmL−1with a detection limit of 6.7pgmL−1and PSA is from 0.01 to 50ngmL−1with a detection limit of 3.4pgmL−1(S/N=3). Importantly, the proposed method offers promise for rapid, simple and cost-effective analysis of biological samples.