Growth and toxin production in the ciguatera-causing dinoflagellate Gambierdiscus polynesiensis (Dinophyceae) in culture
Growth and toxin production in the ciguatera-causing dinoflagellate Gambierdiscus polynesiensis (Dinophyceae) in culture
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DOI：
10.1016/j.toxicon.2009.06.013
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发表时间：
2010-10-01
期刊：
影响因子：
2.8
通讯作者：
Pauillac, Serge
中科院分区：
文献类型：
--
作者：
Chinain, Mireille;Darius, H. Taiana;Pauillac, Serge
在分批培养条件下，研究了一株克隆菌株TB-92的生长和产毒情况。对数生长期的平均生长速率为（0.13 +/- 0.03）分裂日（-1）。无论培养物的年龄如何，注射二氯甲烷和甲醇提取物的所有小鼠均分别表现出对雪卡毒素（CTX）和美托毒素（MTX）生物活性具有特异性的症状。TB-92培养物中评估的最高总毒性为10.4 x 10（-4）小鼠单位细胞（-1）。毒素产生模式揭示了随着培养时间的延长，细胞毒素含量增加。CTX-和MTX-样化合物各自占大约。TB-92培养物的总毒性的50%，除了在CTX占主导地位的老化细胞中。通过受体结合试验，进一步证实了TB-92在二氯甲烷提取物中的高雪卡毒活性。在稳定期晚期监测的最高CTX水平为（11.9 +/- 0.4）pg P-CTX-3C equiv cell（-1）。进一步的HPLC和LC-MS分析显示脂溶性提取物中存在5种CTX同系物，即CTX-3C、-3B、-4A、-4B和M-seco-CTX-3C，以及新的CTX同系物。两种G. polynesiensis菌株表明毒素谱在该物种中是稳定的特征。G.波利尼西亚克隆也被证明比从其他地理区域分离的其他甘比盘属物种具有更高的毒性。(C)2009爱思唯尔有限公司保留所有权利。
The growth and toxin production in a clonal strain of Gambierdiscus polynesiensis, TB-92, was examined in batch culture conditions. The mean growth rate at exponential phase was (0.13 +/- 0.03) division day(-1). Regardless of the age of cultures, all mice injected with dichloromethanolic and methanolic extracts showed symptoms specific to ciguatoxin (CTX) and maitotoxin (MTX) bioactivity, respectively. The highest total toxicity assessed in TB-92 cultures was 10.4 x 10(-4) mouse unit cell(-1). The toxin production pattern reveals an enhanced cellular toxin content with the age of the culture. CTX- and MTX-like compounds each accounted for approx. 50% of the total toxicity of TB-92 cultures, except in aged cells where CTXs were dominant. The high ciguatoxic activity of TB-92 was further confirmed in dichloromethanolic extracts by means of the receptor-binding assay. The highest CTX level monitored at late stationary phase was (11.9 +/- 0.4) pg P-CTX-3C equiv cell(-1). Further HPLC and LC-MS analysis revealed the presence of five CTXs congeners in lipid-soluble extracts, i.e. CTX-3C, -3B, -4A, -4B and M-seco-CTX-3C, and of new CTX congeners. Toxin composition comparison between two G. polynesiensis strains suggests that the toxin profile is a stable characteristic in this species. G. polynesiensis clones also proved inherently more toxic than other Gambierdiscus species isolated from other geographical areas. (C) 2009 Elsevier Ltd. All rights reserved.