Cloning of two new human helicase genes of the RecQ family: Biological significance of multiple species in higher eukaryotes
Cloning of two new human helicase genes of the RecQ family: Biological significance of multiple species in higher eukaryotes
复制标题
DOI：
10.1006/geno.1998.5595
复制
发表时间：
1998-12-15
期刊：
影响因子：
4.4
通讯作者：
Shimamoto, A
中科院分区：
文献类型：
--
作者：
Kitao, S;Ohsugi, I;Shimamoto, A
克隆了两个新的人类DNA解旋酶基因RecQ 4和RecQ 5，它们属于RecQ解旋酶家族。这些基因的加入使人类RecQ家族中的解旋酶基因总数增加到5个，其中包括与Bloom和Werner综合征有关的解旋酶，Bloom和Werner综合征是一种遗传疾病，表现出独特但重叠的免疫缺陷、过早衰老和癌症风险增加的临床表型。RecQ 4解旋酶与Bloom（BLM）和Werner（WRN）解旋酶一样大，并且其基因表达谱是器官特异性的，类似于BLM解旋酶。fn相反，RecQ 5解旋酶具有低分子量，类似于人祖RecQ 1解旋酶，并且在所有检查的器官中表达。所有五种人解旋酶基因在培养的K562白血病细胞和成纤维细胞中表达。同步化的K562细胞培养物显示，RecQ 4和BLM以及RecQ 1和WRN基因似乎分别在细胞周期的G1/S和G2/M期上调。根据单细胞生物如大肠杆菌和酵母只含有一种RecQ解旋酶的事实，讨论了多种RecQ解旋酶的生物学意义。RecQ解旋酶显然不是生命所必需的，但可能与不同的疾病有关。(C)北京：科学出版社.
Two new human DNA helicase genes, RecQ4 and RecQ5, that belong to the RecQ helicase family were cloned and characterized. The addition of these genes increases the total to five helicase genes in the human RecQ family, which includes helicases involved in Bloom and Werner syndromes, the genetic diseases manifesting the distinctive but overlapping clinical phenotypes of immunodeficiency, premature aging, and an enhanced risk of cancer. The RecQ4 helicase is as large as the Bloom (BLM) and Werner (WRN) helicases, and its gene expression profile is organ-specific, resembling that of BLM helicase. fn contrast, the RecQ5 helicase has a low molecular weight, similar to the human progenitor RecQ1 helicase, and is expressed in all the organs examined. All five human helicase genes are expressed in cultured K562 leukemia and fibroblast cells. Synchronized K562 cell cultures showed that the genes RecQ4 and BLM, and RecQ1 and WRN, seem to be upregulated at the G1/S and G2/M phases, respectively, of the cell cycle. The biological significance of multiple species of human RecQ helicases, which are apparently nonessential for Life but may be related to distinct diseases, is discussed in light of the fact that unicellular organisms, Like Escherichia coli and yeast, contain only one species of helicase of this particular family. (C) 1998 Academic Press.