Embryonic Alcohol Exposure Impairs the Dopaminergic System and Social Behavioral Responses in Adult Zebrafish
Embryonic Alcohol Exposure Impairs the Dopaminergic System and Social Behavioral Responses in Adult Zebrafish
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DOI：
10.1093/ijnp/pyu089
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发表时间：
2015-04-01
影响因子：
4.8
通讯作者：
Gerlai, Robert
中科院分区：
文献类型：
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作者：
Fernandes, Yohaan;Rampersad, Mindy;Gerlai, Robert
背景资料：斑马鱼是一种强大的神经行为遗传学工具，可以模拟和研究复杂的人类大脑疾病，包括酒精滥用和胎儿酒精谱系障碍。斑马鱼天生就形成了一种叫做鱼群的社会群体。以前，已经证明，单浴暴露（受精后24小时）至低剂量酒精（0、0.25、0.50、0.75和1%vol/vol）持续短时间（2小时）导致成年斑马鱼的群体形成或变浅受损。在目前的研究中，我们将斑马鱼卵在受精后24小时浸入低浓度酒精（0.5%或1%vol/vol）中2小时，让鱼生长并达到成年。除了定量的行为反应的成年鱼的动画浅滩，我们还测量了多巴胺及其代谢产物3，4-dihydroxyphenylacetic acid从全脑提取物的这些鱼使用高压液相chromatography.Results：在这里，我们证实，胚胎酒精暴露使成年斑马鱼增加他们的距离，以剂量依赖的方式从浅滩刺激。我们还表明，浅滩刺激增加控制斑马鱼的大脑中的多巴胺和3，4-二羟基苯乙酸的量，但不是在鱼以前暴露于酒精在其胚胎development.Conclusions：我们推测的机制之一，可以解释胚胎酒精诱导受损的浅滩反应在斑马鱼是由多巴胺能系统的奖励机制subserved功能障碍。
Background: The zebrafish is a powerful neurobehavioral genetics tool with which complex human brain disorders including alcohol abuse and fetal alcohol spectrum disorders may be modeled and investigated. Zebrafish innately form social groups called shoals. Previously, it has been demonstrated that a single bath exposure (24 hours postfertilization) to low doses of alcohol (0, 0.25, 0.50, 0.75, and 1% vol/vol) for a short duration (2 hours) leads to impaired group forming, or shoaling, in adult zebrafish.Methods: In the current study, we immersed zebrafish eggs in a low concentration of alcohol (0.5% or 1% vol/vol) for 2 hours at 24 hours postfertilization and let the fish grow and reach adulthood. In addition to quantifying the behavioral response of the adult fish to an animated shoal, we also measured the amount of dopamine and its metabolite 3,4-dihydroxyphenylacetic acid from whole brain extracts of these fish using high-pressure liquid chromatograph.Results: Here we confirm that embryonic alcohol exposure makes adult zebrafish increase their distance from the shoal stimulus in a dose-dependent manner. We also show that the shoal stimulus increases the amount of dopamine and 3,4-dihydroxyphenylacetic acid in the brain of control zebrafish but not in fish previously exposed to alcohol during their embryonic development.Conclusions: We speculate that one of the mechanisms that may explain the embryonic alcohol-induced impaired shoaling response in zebrafish is dysfunction of reward mechanisms subserved by the dopaminergic system.