A SENSITIVE AND SPECIFIC DNA PROBE TO IDENTIFY ENTEROAGGREGATIVE ESCHERICHIA-COLI, A RECENTLY DISCOVERED DIARRHEAL PATHOGEN
A SENSITIVE AND SPECIFIC DNA PROBE TO IDENTIFY ENTEROAGGREGATIVE ESCHERICHIA-COLI, A RECENTLY DISCOVERED DIARRHEAL PATHOGEN
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DOI：
10.1093/infdis/161.6.1249
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发表时间：
1990-06-01
影响因子：
6.4
通讯作者：
LEVINE, MM
中科院分区：
文献类型：
--
作者：
BAUDRY, B;SAVARINO, SJ;LEVINE, MM
肠聚集性大肠杆菌（EAggEC）作为一种肠道病原体的流行病学意义只是最近才开始研究。虽然EAggEC与一些发展中国家婴儿的持续腹泻有关，但显然需要进行更多的研究。到目前为止，鉴定EAggEC菌株的唯一方法是繁琐的HEp-2细胞粘附试验。本文报道了从EAggEC 17-2株质粒中分离和克隆1-乙酰氨基转移酶片段。该探针对EAggEC的敏感性为89%，特异性为99%。因此，这种探针应大大有助于流行病学研究评估EAggEC作为一种肠道病原体的重要性。
The epidemiologic significance of enteroaggregative Escherichia coli (EAggEC) as a diarrheal pathogen has only recently come under study. Although EAggEC has been associated with persistent diarrhea in infants in some developing countries, additional studies are clearly needed. Until now, the only means of identifying EAggEC strains has been the cumbersome HEp-2 cell adhesion assay. The isolation and cloning of a 1-kilobase fragment from the plasmid of EAggEC strain 17-2 is described. This probe is 89% sensitive and 99% specific for EAggEC identification. Thus, this probe should greatly facilitate epidemiologic studies assessing the importance of EAggEC as a diarrheal pathogen.