Use of 123I and 14C in a double radionuclide autoradiographic technique for simultaneous measurement of LCBF and LCMRgl. Theory and method.
Use of 123I and 14C in a double radionuclide autoradiographic technique for simultaneous measurement of LCBF and LCMRgl. Theory and method.
复制标题
在双放射性核素放射自显影技术中使用 123I 和 14C 同时测量 LCBF 和 LCMRgl。
DOI：
10.1161/01.str.12.5.589
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发表时间：
1981
期刊：
影响因子：
8.3
通讯作者：
Reivich,M
中科院分区：
文献类型：
--
作者：
Lear,JL;Jones,SC;Greenberg,JH;Fedora,TJ;Reivich,M
我们开发了一种放射自显像技术，分别使用123i -碘安替比林和14c -2脱氧葡萄糖同时测量局部脑血流量（LCBF）和局部脑葡萄糖代谢率（LCMRgl）。通过利用123I和14C不同的半衰期（13.0小时和5730年），我们制作了2张自放射成像图像。第一个主要来自123I，第二个主要来自14C。由于制定123I标准的不可行性，有必要确定与123I相对于14C曝光柯达SB-54胶片的能力有关的常数，以便使用14C标准来量化123I曝光。然后可以用减法方程来解出局部的123I和14C浓度。该技术在模拟流量和代谢12倍解耦的条件下进行了验证，测量示踪剂浓度的标准偏差小于6%。然后用它在正常清醒的大鼠中测量这些参数，得到的值与发表的单一放射性核素研究的值吻合得很好。该技术有望用于同时测量各种生理或病理状态下的LCBF和LCMRgl，包括那些血流和代谢明显解耦的状态。此外，通过使用标记为123I和14C的其他示踪剂，可以同时测量其他参数。
We have developed an autoradiographic technique for the simultaneous measurement of local cerebral blood flow (LCBF) and local cerebral metabolic rate for glucose (LCMRgl) using 123I-iodoantipyrine and 14C-2-deoxyglucose respectively. By exploiting the different half-lives of 123I and 14C, 13.0 hours versus 5730 years, we produce 2 autoradiographic images. The first is predominantly a result of the 123I and the second is predominantly from the 14C. Because of the impracticality of making 123I standards, it was necessary to determine the constant which relates the ability of 123I, with respect to 14C, to expose Kodak SB-54 film, so that the 123I exposure could be quantified using 14C standards. Subtraction equations can then be used to solve for the local 123I and 14C concentrations. The technique was validated in conditions simulating a 12-fold decoupling of flow and metabolism and the standard deviation of error in measuring tracer concentrations was less than 6%. It was then used to measure these parameters in the normal awake rats and values obtained agreed well with published values from single radionuclide studies. The technique is expected to be useful in the simultaneous measurement of LCBF and LCMRgl in various physiologic or pathologic states, including those with significant decoupling of flow and metabolism. In addition, by using other tracers labelled with 123I and 14C, other parameters can be measured concurrently.