Rapid determination of the dry weight of single, living cyanobacterial cells using the Mach-Zehnder double-beam interference microscope
Rapid determination of the dry weight of single, living cyanobacterial cells using the Mach-Zehnder double-beam interference microscope
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DOI：
10.1080/09670260802168625
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发表时间：
2008-01-01
影响因子：
2.4
通讯作者：
Loosen, Peter
中科院分区：
文献类型：
--
作者：
Mahlmann, Daniel M.;Jahnke, Joachim;Loosen, Peter
透射双光束干涉显微镜被用来确定干重每单位生物体积的单个活细胞，毛状体和粘液鞘的8个主要陆地物种的蓝藻从文化和原位样品。最小干重为131.7 fg m-3，而最大值为459.2 fg m-3，来自单电池测量。所有72个测量值的平均值（SD）为265 - 46 fg m-3。该值比根据文献数据计算的平均值低1.8倍。额外的元素测量的碳量导致的平均值（SD）的48 - 3%的干重，这与文献数据相对应。因此，我们建议一个新的转换因子为0.127的生物体积（毫米3）的细胞毫克碳，这可用于蓝藻在整体生物量计算。还对毛状体（席藻属）和粪菌（粘球藻属）的粘液鞘进行了干重测量。鞘的单位体积干重变化很大，从28 fg m-3（席藻属）到70 fg m-3，甚至210 fg m-3（粘球藻属）。在Gloeocapsa鞘材料的一个单一的coenobium超过细胞干重6倍的干质量。由于干涉显微镜技术在确定单个未处理活细胞的干重方面具有独特的能力，即使在复杂的环境样品中，我们也打算开发这种方法，使其可用于广泛的应用。
The transmitted double-beam interference microscope was used to determine the dry weight per unit biovolume of single living cells, trichomes and mucous sheaths of eight mainly terrestrial species of cyanobacteria from cultures and in situ samples. The minimum dry weight was 131.7 fg m-3 whereas the maximum was 459.2 fg m-3 from single cell measurements. The average (SD) of all 72 measurements was 265 46 fg m-3. This value is lower than the average calculated from literature data by a factor of 1.8. Additional elemental measurements of the amount of carbon resulted in an average value (SD) of 48 3% of dry weight, which corresponds with literature data. Thus we recommend a new conversion factor of 0.127 for biovolume (mm3) of cells to mg carbon, which could be used for cyanobacteria in respect to overall biomass calculations. Dry weight measurements were also carried out on the mucous sheaths of both trichomes (Phormidium) and coenobia (Gloeocapsa). Dry weights per unit volume of the sheaths varied greatly, ranging from 28 fg m-3 (Phormidium) to 70 fg m-3 and even 210 fg m-3 (Gloeocapsa). In Gloeocapsa the dry mass of sheath material of a single coenobium exceeded the cellular dry weight 6-fold. As the interference microscopical technique is unique in its ability to determine dry masses of single living untreated cells, even in complex environmental samples, we intended to develop this method to make it available to a broad range of applications.