SEPARATION AND DETECTION OF DNA BY CAPILLARY ELECTROPHORESIS
SEPARATION AND DETECTION OF DNA BY CAPILLARY ELECTROPHORESIS
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DOI：
10.1016/s0021-9673(00)90574-0
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发表时间：
1988-12-23
期刊：
影响因子：
--
通讯作者：
BROWNLEE, RG
中科院分区：
文献类型：
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作者：
KASPER, TJ;MELERA, M;BROWNLEE, RG
The use of capillary electrophoresis for the separation and detection of nucleic acids has been investigated. Lab-model instruments have been built, using commercially available UV absorbance and fluorescence detectors which were modified for use with 50-100 μm I.D. fused-silica capillary tubing. The sensitivity of these instruments (signal-to-noise ratio = 3) was measured as 15 μg/ml for fluorescence detection of ethidium bromide-stained herring-sperm DNA and 3 smg/ml for UV absorbance detection. With the former instruments a variety of strategies has been used to attain rapid separations of bases, oligonucleotides, restriction fragments and whole phage, viral and plasmid DNAs.
