High-resolution genetic mapping of the pepper (Capsicum annuum L.) resistance loci Me3 and Me4 conferring heat-stable resistance to root-knot nematodes (Meloidogyne spp.)
High-resolution genetic mapping of the pepper (Capsicum annuum L.) resistance loci Me3 and Me4 conferring heat-stable resistance to root-knot nematodes (Meloidogyne spp.)
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DOI：
10.1007/pl00002914
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发表时间：
2001-09-01
影响因子：
5.4
通讯作者：
Abad, P
中科院分区：
文献类型：
--
作者：
Djian-Caporalino, C;Pijarowski, L;Abad, P
辣椒PM 687系是一个优良的辣椒品系。具有单一显性基因Me-3，其赋予对根结线虫（RKN）的热稳定抗性。利用感病品种YoloWonder（YW）与高抗品系PM 687杂交的双单倍体（DH）系和F-2后代对Me-3进行定位。用感病或抗病DH系的DNA池进行批量分离分析，以确定与Me-3连锁的RAPD和AFLP标记。使用800多个RADP引物（分析了4，000个扩增片段），10个DH系的批量之间没有多态性。使用512个AFLP引物（分析了74，000个扩增片段）和来自20个抗性和20个感病植物的大量DNA模板，我们确定了与Me-3连锁的8个排斥期和4个偶联期标记。在103个DH后代中分析，他们确定了含有目的基因的56.1-cM区间。最近的距离分别位于基因两侧的0.5、1.0、1.5和3.0厘摩（cM）。对具有最近的偶联相标记的F-2后代（162株植物）的分析证实了其接近的位置。另外一个抗RKN基因（Me-4）存在于‘PM 687’中，与Me-3连锁，距离其10 cM。Me-3与RAPD标记Q04_0.3的距离为10.1cM，与RFLP标记CT 135的距离为2.7cM。我们研究了Me-3和另外两个线虫抗性基因（番茄Mi-3和马铃薯Gpa（2）基因）之间的图位同源性，这两个基因定位在番茄和马铃薯12号染色体短臂的端粒区（或马铃薯的XII）。
The PM687 line of Capsicum annuum L. has a single dominant gene, Me-3, that confers heat-stable resistance to root-knot nematodes (RKN). Me-3 was mapped using doubled-haploid (DH) lines and F-2 progeny from a cross between the susceptible cultivar 'Yolo Wonder' ('YW') and the highly resistant line 'PM687'. Bulked-segregant analysis with DNA pools, from susceptible or resistant DH lines, was performed to identify RAPD and AFLP markers linked to Me-3. There was no polymorphism between bulks of ten DH lines using over 800 RADP primers (4,000 amplified fragments analysed). Using 512 AFLP primers (74,000 amplified fragments analysed), and bulked DNA templates from 20 resistant and 20 susceptible plants, we identified eight repulsion-phase and four coupling-phase markers linked to Me-3. Analysed in 103 DH progeny, they defined a 56.1-cM interval containing the target gene. The nearest were located 0.5, 1.0, 1.5 and 3.0 centimorgans (cM) on both sides of the gene. Analysis of the F-2 progeny (162 plants) with the nearest coupling-phase marker confirmed its close position. Another resistance gene to RKN, present in 'PM687' (Me-4), was shown to be linked to Me-3, 10 cM from it. In order to localize Me-3 and Me-4 on our reference intraspecific pepper linkage map, two AFLP markers were mapped. The Me-3 nearest marker was 10,1 cM from a RAPD marker named Q04_0.3 and 2.7 cM from a RFLP marker named CT135. We investigated map-position orthologies between Me-3 and two other nematode resistance genes, the tomato Mi-3 and the potato Gpa(2) genes, which mapped in the telomeric region of the short arm of the tomato and potato chromosome 12 (or XII for potato).