Comprehensive phenotypic analysis for identification of genes affecting growth under ethanol stress in Saccharomyces cerevisiae
Comprehensive phenotypic analysis for identification of genes affecting growth under ethanol stress in Saccharomyces cerevisiae
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DOI：
10.1111/j.1567-1364.2008.00456.x
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发表时间：
2009-02-01
影响因子：
3.2
通讯作者：
Shimizu, Hiroshi
中科院分区：
文献类型：
--
作者：
Yoshikawa, Katsunori;Tanaka, Tadamasa;Shimizu, Hiroshi
我们量化了所有可用的单基因缺失芽殖酵母菌株在乙醇胁迫下的生长行为。全基因组分析能够提取基因并确定在此条件下生长所需的功能类别。统计分析表明，446株缺失菌株在8%乙醇胁迫下生长出现缺陷。我们将这些删除的基因分类为已知的功能类别，发现许多基因对于乙醇胁迫下的生长很重要，包括一些尚未表征的类别，例如过氧化物酶体。我们还在渗透胁迫下进行了全基因组筛选，鉴定了 329 个渗透敏感菌株。我们从 446 个乙醇敏感菌株中排除了这些菌株，以提取因删除而导致对乙醇特异性（359 个基因）、渗透特异性（242 个基因）和两种应激（87 个基因）敏感的基因。我们还提取了对乙醇胁迫下的生长特别重要的功能类别。分析中确定的基因和功能类别可能为提高酵母细胞的乙醇胁迫耐受性提供线索。
We quantified the growth behavior of all available single gene deletion strains of budding yeast under ethanol stress. Genome-wide analyses enabled the extraction of the genes and determination of the functional categories required for growth under this condition. Statistical analyses revealed that the growth of 446 deletion strains under stress induced by 8% ethanol was defective. We classified these deleted genes into known functional categories, and found that many were important for growth under ethanol stress including several categories that have not been characterized, such as peroxisome. We also performed genome-wide screening under osmotic stress and identified 329 osmotic-sensitive strains. We excluded these strains from the 446 ethanol-sensitive strains to extract the genes whose deletion caused sensitivity to ethanol-specific (359 genes), osmotic-specific (242 genes), and both stresses (87 genes). We also extracted the functional categories that are specifically important for growth under ethanol stress. The genes and functional categories identified in the analysis might provide clues to improving ethanol stress tolerance among yeast cells.