Genomic characterization of Listeria monocytogenes isolates reveals that their persistence in a pig slaughterhouse is linked to the presence of benzalkonium chloride resistance genes
Genomic characterization of Listeria monocytogenes isolates reveals that their persistence in a pig slaughterhouse is linked to the presence of benzalkonium chloride resistance genes
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DOI：
10.1186/s12866-018-1363-9
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发表时间：
2018-12-20
期刊：
影响因子：
4.2
通讯作者：
Fravalo, Philippe
中科院分区：
文献类型：
--
作者：
Cherifi, Tamazight;Carrillo, Catherine;Fravalo, Philippe
BackgroundThe的目的是本研究的特征的基因组的30 L单核细胞增生李斯特菌菌株收集在猪屠宰场，以确定其持久性的分子基础。单核细胞增多症基因组显示连续的分离株（即，持久型）可以基于局限于原噬菌体区域的单核苷酸变异体进行关联。此外，我们的研究揭示了这些菌株中存在bcrABC盒，已知该盒可产生外排泵介导的苯扎氯铵耐药性，这可能是这些分离株在屠宰场环境中持续存在的原因。通过WGS和PCR确认bcrABC盒的存在，并通过测量最小抑制浓度确定耐药表型。此外，BC-耐药菌株被发现产生较低的生物膜量的存在下，亚致死浓度的BC。ConclusionsHigh resolution SNP为基础的分型和确定的bcrABC盒可能提供一种手段，居民和散发L。这将有助于在食品工业中更好地管理这种病原体。
BackgroundThe aim of this study was to characterize the genomes of 30Listeria monocytogenes isolates collected at a pig slaughterhouse to determine the molecular basis for their persistence.ResultsComparison of the 30L. monocytogenes genomes showed that successive isolates (i.e., persistent types) recovered from thew sampling site could be linked on the basis of single nucleotide variants confined to prophage regions. In addition, our study revealed the presence among these strains of the bcrABC cassette which is known to produce efflux pump-mediated benzalkonium chloride resistance, and which may account for the persistence of these isolates in the slaughterhouse environment. The presence of the bcrABC cassette was confirmed by WGS and PCR and the resistance phenotype was determined by measuring minimum inhibitory concentrations. Furthermore, the BC-resistant strains were found to produce lower amounts of biofilm in the presence of sublethal concentrations of BC.ConclusionsHigh resolution SNP-based typing and determination of the bcrABC cassette may provide a means of distinguishing between resident and sporadic L. monocytogenes isolates, and this in turn will support better management of this pathogen in the food industry.