LEPTOSPIRA INTERROGANS ENTERS DIFFERENT HOST CELLS BY BINDING TO DISTINCT MOLECULES IN THE EXTRACELLULAR MATRIX
LEPTOSPIRA INTERROGANS ENTERS DIFFERENT HOST CELLS BY BINDING TO DISTINCT MOLECULES IN THE EXTRACELLULAR MATRIX
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DOI：
10.2298/abs140702003z
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发表时间：
2015
影响因子：
0.8
通讯作者：
Xin Zhao;Aihua Sun;Yu-Mei Ge;Huanping Wang;Jie Yan
中科院分区：
文献类型：
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作者：
Xin Zhao;Aihua Sun;Yu-Mei Ge;Huanping Wang;Jie Yan
钩端螺旋体病是由钩端螺旋体引起的一种世界性的人畜共患病。2螺旋体能有效地侵入宿主细胞并从血液扩散到内脏器官，但钩体内化到不同宿主细胞的机制仍知之甚少。钩端螺旋体与ECM（细胞外基质）的粘附启动了巨噬细胞系THP-1、J774A.1和人脐上皮细胞（HUVEC）中钩端螺旋体的内化。我们评估了不同细胞中ECM的分布，包括纤连蛋白（FN）、层粘连蛋白和胶原蛋白1-4（COL 1-COL 4）。FN在THP-1和J774A.1细胞中表达，FN、LN、COL 3和COL 4在HUVEC中表达。然后，我们发现钩端螺旋体粘附FN，LN，COL 3和COL 4。当用FN预封闭钩体时，巨噬细胞（THP-1和J774 A）中最重要的细胞内钩体显著减少。这表明FN在钩体在巨噬细胞中的粘附和进入中具有重要作用，而在HUVEC中，FN、LN、COL 3和COL 4承担这种作用。
Leptospirosis caused by Leptospira is a worldwide zoonotic disease. The 2 spirochete can efficiently invade host cells and spread from blood into internal organs, but the mechanisms of leptospiral internalization into different host cells remain poorly understood. Adhesion of leptospires to the ECM (extracellular matrix) initiated leptospire internalization in macrophage cell-lines THP-1, J774A.1, and in human umbilical epithelial cells (HUVEC). We assessed the distribution of ECM , including fibronectin (FN), laminin and collagens 1-4 (COL1-COL4) in different cells. FN was expressed in THP-1 and J774A.1 cells, and FN, LN, COL3 and COL4 were expressed in HUVEC. Then, we found leptospires adhered to FN, LN, COL3 and COL4. The most important intracellular leptospires in macrophages (THP-1 and J774A) significantly decreased when leptospires were pre-blocked with FN This indicates that FN has an important role in the adherence to and entry of leptospires in macrophages, while in HUVEC, FN, LN, COL3 and COL4 assume this role.