Identification of microRNAs responding to cold stress in Dongxiang common wild rice

Identification of microRNAs responding to cold stress in Dongxiang common wild rice
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东乡野生稻冷胁迫响应的 microRNA 鉴定

DOI:
10.1139/gen-2019-0015
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发表时间:
2019-09-01
期刊:
影响因子:
3.1
通讯作者:
Liu, Fengxia
Liu, Fengxia
中科院分区:
生物学3区
文献类型:
--
作者:
Jiang, Wanxia;Shi, Wenkuan;Liu, Fengxia

文献摘要

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低温是水稻不同生育阶段的重要效应因子。 MicroRNA (miRNA) 在应对非生物和生物胁迫方面发挥着重要作用。在这里,我们证实了东乡野生稻的耐冷性,并利用全基因组小RNA测序探索了冷胁迫下差异表达的miRNA。总共在东乡野生稻中鉴定了 16 个 miRNA,其中 9 个在冷胁迫下上调,7 个下调,并预测了它们的靶基因。此外,对目标基因的 AgriGO 分析显示,它们富含与冷胁迫耐受性相关的多个术语,表明东乡普通野生稻对冷胁迫存在复杂的响应机制,涉及 miRNA。
Low temperature is a vital effector of rice at different growth stages. MicroRNAs (miRNAs) play important roles in responding to abiotic and biotic stresses. Here, we confirmed the cold tolerance of Dongxiang common wild rice and explored the miRNAs differentially expressed under cold stress using genome-wide small RNA sequencing. In total, 16 miRNAs, nine upregulated and seven downregulated by cold stress, were characterized in Dongxiang common wild rice, and their target genes were predicted. Additionally, an AgriGO analysis of the target genes revealed that they were enriched in several terms related to cold-stress tolerance, suggesting a complex response mechanism, involving miRNAs, to cold stress in Dongxiang common wild rice.