The growth of a vascular network inside a collagen-citric acid derivative hydrogel in rats
The growth of a vascular network inside a collagen-citric acid derivative hydrogel in rats
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DOI：
10.1016/j.biomaterials.2009.03.026
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发表时间：
2009-07-01
期刊：
影响因子：
14
通讯作者：
Nagawa, Hirokazu
中科院分区：
文献类型：
--
作者：
Takayama, Toshio;Taguchi, Tetsushi;Nagawa, Hirokazu
具有一定体积的三维再生组织在体内没有足够的血液灌注就无法存活，因此在再生组织中构建血管系统是组织工程的关键技术。为了构建这样的血管系统，我们试图创建一种支架材料，其诱导从受体床到材料中的新血管生长。这种材料是由胶原蛋白和柠檬酸衍生物组成的离子复合物凝胶基质（IC凝胶），使其能够与碱性成纤维细胞生长因子（bFGF）结合。将IC凝胶植入大鼠直肌筋膜下间隙，5天后切除。将切下的样品的横截面进行血管性血友病因子染色，然后观察凝胶中的新生血管发育，并通过图像分析进行定量。这些数据表明，IC凝胶在第5天显著诱导富含血管的组织生长到凝胶内部，这超过了植入Matrigel（R）或凝胶化胶原后的情况。此外，与bFGF的组合显著增强IC凝胶的血管化能力。这些结果表明，IC凝胶作为一种支架材料的新生血管长入和bFGF的水库。(C)2009爱思唯尔有限公司保留所有权利。
Three-dimensional regenerative tissue with a certain bulk cannot survive without sufficient blood perfusion in vivo, so construction of a vascular system in regenerative tissue is a key technology in tissue engineering. In order to construct such a vascular system, we attempted to create a scaffold material that induces neovascular growth from the recipient bed into the material. This material, an ion complex gel matrix (IC gel) consisting of collagen and a citric acid derivative, enabled it to associate with basic fibroblast growth factor (bFGF). The IC gel was implanted in the subfascial space of the rat rectus muscle and excised 5 days later. Cross-sections of the excised samples were stained for von Willebrand factor, and then neovascular development into the gel was observed and also quantified by image analysis. These data showed that the IC gel markedly induced growth of vascular-rich tissue into the inside of the gel by day 5, which surpassed that after implantation of Matrigel (R) or gelated collagen. Further, combination with bFGF significantly enhanced the vascularization ability of IC gel. These findings suggest that IC gel functioned as a scaffold material for neovascular ingrowth and a reservoir of bFGF. (C) 2009 Elsevier Ltd. All rights reserved.