OXYGEN-INDUCED CHANGES IN PULMONARY SUPEROXIDE-DISMUTASE ASSAYED BY ANTIBODY TITRATIONS
OXYGEN-INDUCED CHANGES IN PULMONARY SUPEROXIDE-DISMUTASE ASSAYED BY ANTIBODY TITRATIONS
复制标题
DOI：
10.1152/ajplegacy.1976.231.4.1196
复制
发表时间：
1976-01-01
影响因子：
--
通讯作者：
MCCORD, JM
中科院分区：
文献类型：
--
作者：
CRAPO, JD;MCCORD, JM
先前的研究表明，O2 适应大鼠的肺超氧化物歧化酶 (SOD) 活性增加了 50%，并表明 SOD 在耐受性的发展中发挥着重要作用。为了进一步研究这些事件，从大鼠肝脏中纯化了铜锌 SOD，发现其与之前纯化的铜锌超氧化物歧化酶相似。制备了针对大鼠铜锌 SOD 的兔抗血清，并用于对暴露于 85% O2 5 天的大鼠肺部进行 SOD 抗体滴定。通过抗体滴定，铜锌 SOD 的绝对量增加了 41%，这占总 SOD 活性 48% 增加的大部分（如果不是全部）。在 pH 7.8 和 10.0 下对纯大鼠铜锌 SOD 和粗制肺匀浆进行分光光度测定表明，还存在锰 SOD，但该 SOD 在 O2 耐受性发展中的作用尚未确定。
Previous studies demonstrated a 50% increase in pulmonary superoxide dismutase (SOD) activity in O2-adapted rats and suggested that SOD plays a significant role in the development of tolerance. To further study these events, the cuprozinc SOD was purified from rat liver and found to be similar to previously purified cuprozinc superoxide dismutases. A rabbit antisera to rat cuprozinc SOD was produced and used to perform antibody titrations of SOD in the lungs of rats exposed to 85% O2 for 5 days. The absolute amount of cuprozinc SOD increased 41% by antibody titration which accounted for most, if not all, of the 48% increase demonstrated in total SOD activity. Spectrophotometric assays at pH 7.8 and 10.0 of pure rat cuprozinc SOD and crude lung homogenates suggest that there is also a manganese SOD present, but the role of that SOD in the development of O2 tolerance has not been established.