Enhancement of the in vitro infectivity of a nuclear polyhedrosis virus by a factor in the capsule of a granulosis virus
Enhancement of the in vitro infectivity of a nuclear polyhedrosis virus by a factor in the capsule of a granulosis virus
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颗粒病毒荚膜中的因子增强核多角体病毒的体外感染性
DOI：
10.1016/0022-2011(89)90142-0
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发表时间：
1989
影响因子：
3.4
通讯作者：
Yafeng Zhu
中科院分区：
文献类型：
--
作者：
T. Hukuhara;Yafeng Zhu
在棉铃虫核型多角体病毒(TnNPV)质膜萌芽病毒和多角体病毒(TnNPV)的同源细胞系中，存在协同因子(SYF)，可促进Spod聚果蝇细胞(IPLB-SF21AE)的侵染。SYF与表面OFS的特异性结合。果树核型多角体病毒(TnNPV)和核型多角体病毒(P)的囊膜。分别用免疫荧光显微镜和免疫电子显微镜观察单核NPV。SYF对B系细胞无特异性亲和力。Mori(BM-N)、分离性假单胞菌(NIAS-LESE-11)和T.Ni(TN-368)或B的多面体来源的病毒粒子。MoriNPV。抗SYF抗体和氯喹可抑制SYF的活性。
A synergistic factor (SyF) present in the capsules of the Hawaiian strain ofPseudaletia unipunctagranulosis virus enhanced the infection ofSpodopera frugiperdacells (IPLB-SF21AE) in culture with plasma-membrane-budded and polyhedra-derived virions ofTrichoplusia nimultinucleocapsid nuclear polyhedrosis virus (TnNPV).Bombyx moriNPV,P. unipunctaNPV, and TnNPV, when assayed in their homologous cell lines in the presence of the SyF, were not enhanced in their infectivity. Specific binding of the SyF to the surface ofS. frugiperdacells and to the envelopes of TnNPV andP. unipunctaNPV was visualized by immunofluorescence microscopy and immunoelectron microscopy, respectively. The SyF had no specific affinity to cell lines fromB. mori(BM-N),P. separata(NIAS-LeSe-11), andT. ni(TN-368) or to polyhedra-derived virions ofB. moriNPV. Anti-SyF antibodies and chloroquine inhibited the activity of the SyF.