Development and Application of Zirconia Coated Paper Substrate for High Sensitivity Analysis of Therapeutic Drugs in Dried Blood Spots
Development and Application of Zirconia Coated Paper Substrate for High Sensitivity Analysis of Therapeutic Drugs in Dried Blood Spots
复制标题
氧化锆涂层纸基片在干血斑治疗药物高灵敏度分析中的开发与应用
DOI：
10.1021/acs.analchem.5b04732
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发表时间：
2016-07-19
影响因子：
7.4
通讯作者：
Zhang, Zhiping
中科院分区：
文献类型：
--
作者：
Zheng, Yajun;Wang, Qian;Zhang, Zhiping
纸喷雾质谱法已被证明有望用于直接分析干血斑 (DBS) 中的治疗药物；然而，纸张基材和含有极性官能团的分析物（例如治疗药物）之间的强氢键和范德华相互作用极大地影响纸张喷雾过程中目标化合物的洗脱行为和分析灵敏度。在此，我们使用商业ZrO2颗粒作为涂层材料和可溶性淀粉作为粘合剂，通过简便的真空过滤方法开发了一种单面ZrO2涂层纸基材。由于独特的表面特性，所制备的 ZrO2 纸基质已被证明在纸喷雾质谱法 DBS 中分析治疗药物方面具有优异的性能。与原始纤维素纸基材相比，使用 ZrO2 涂层纸进行纸喷雾，测试药物的定量下限 (LLOQ) 提高了 43-189 倍。与之前报道的SG81级纸和单面二氧化硅涂布纸相比，使用所制备的ZrO2纸测试药物的LLOQ比上述两者下降了1.5-16.5倍，表明ZrO2涂布纸是DBS治疗药物高灵敏度分析中纸喷雾的良好候选者。
Paper spray mass spectrometry has been demonstrated to be promising for direct analysis of therapeutic drugs in dried blood spots (DBS); however, the strong hydrogen bond and van de Waals interactions between paper substrate and analytes containing polar functional groups (e.g., therapeutic drugs) affect greatly the elution behavior and analysis sensitivity of compounds of interest during paper spray. Herein, we developed a one-sided ZrO2 coated paper substrate through a facile vacuum filtration approach using commercial ZrO2 particles as coating material and soluble starch as adhesive agent. Owing to the unique surface properties, as-prepared ZrO2 paper substrate has been shown to have excellent performance for analysis of therapeutic drugs in DBS during paper spray mass spectrometry. In contrast to original cellulose paper substrates, improvements of 43-189-fold in lower limit of quantitation (LLOQ) were obtained for the tested drugs using ZrO2 coated paper for paper spray. In comparing with the previously reported grade SG81 paper and one-sided silica coated paper, the LLOQs of the tested drugs with as-prepared ZrO2 paper decreased 1.5-16.5-fold relative to those from the above two, revealing that ZrO2 coated paper is a good candidate for paper spray in high sensitivity analysis of therapeutic drugs in DBS.