Visualizing mammalian brain area interactions by dual-axis two-photon calcium imaging.
Visualizing mammalian brain area interactions by dual-axis two-photon calcium imaging.
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DOI：
10.1038/nn.3867
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发表时间：
2014-12
影响因子：
25
通讯作者：
Schnitzer MJ
中科院分区：
文献类型：
--
作者：
Lecoq J;Savall J;Vučinić D;Grewe BF;Kim H;Li JZ;Kitch LJ;Schnitzer MJ
荧光Ca2+成像能够大规模记录神经活动，但哺乳动物大脑区域的集体动态通常在单一视野内无法进入。在这里，我们介绍了一种双光子显微镜，它具有两个关节臂，可以同时成像两个大脑区域（每个约0.38 mm2），无论是附近还是远端，使用显微内窥镜。行为小鼠初级视觉皮层（V1）和侧内侧视觉区（LM）约100-300个神经元的同时Ca2+成像显示，LM神经元视觉反应的变异性强烈依赖于V1，表明感觉反应的波动通过扩展的皮质网络传播。
Fluorescence Ca2+ imaging enables large-scale recordings of neural activity, but collective dynamics across mammalian brain regions are generally inaccessible within single fields of view. Here we introduce a two-photon microscope possessing two articulated arms that can simultaneously image two brain areas (~0.38 mm2 each), either nearby or distal, using microendoscopes. Concurrent Ca2+ imaging of ~100–300 neurons in primary visual cortex (V1) and lateromedial (LM) visual area in behaving mice revealed that the variability in LM neurons’ visual responses was strongly dependent on that in V1, suggesting that fluctuations in sensory responses propagate through extended cortical networks.