A 90° rotation of the mitotic spindle changes the orientation of mitoses of zebrafish neuroepithelial cells
A 90° rotation of the mitotic spindle changes the orientation of mitoses of zebrafish neuroepithelial cells
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DOI：
10.1242/dev.00603
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发表时间：
2003-08-01
期刊：
影响因子：
4.6
通讯作者：
Campos-Ortega, JA
中科院分区：
文献类型：
--
作者：
Geldmacher-Voss, B;Reugels, AM;Campos-Ortega, JA
在斑马鱼胚胎躯干区的神经板和神经管中，分裂细胞的方向平行于神经上皮的平面，而在神经龙骨/杆中，细胞垂直于神经上皮的平面分裂。有丝分裂方向的这种变化是由有丝分裂纺锤体旋转90度引起的。由于龙骨/杆阶段的神经原基的两半是并置的，在这个阶段中有丝分裂的垂直方向决定了子细胞被分配到神经管的两侧。为了评估细胞连接在控制分裂细胞方向中所起的作用，我们研究了神经上皮细胞中粘附和紧密连接成分的表达。我们发现，这些蛋白质分布不规则的神经板阶段，并成为极化顶端的细胞膜，只有在龙骨/杆阶段。有丝分裂的定型方向扰动只有微弱的细胞连接组件ASIP和aPKClambda的功能丧失后，这表明有丝分裂的方向部分取决于细胞连接的完整性和作为一个整体的上皮的极性。然而，纺锤体的90度旋转并不需要神经上皮细胞之间的完美极化细胞连接。
In the neural plate and neural tube in the trunk region of the zebrafish embryo, dividing cells are oriented parallel to the plane of the neuroepithelium, while in neural keel/rod, cells divide perpendicular to it. This change in the orientation of mitosis is brought about by a 90degrees rotation of the mitotic spindle. As the two halves of the neural primordium in keel/rod stage are in apposition, the perpendicular orientation of mitoses in this stage determines that daughter cells become allocated to both sides of the neural tube. To assess the role played by cell junctions in controlling the orientation of dividing cells, we studied the expression of components of adherens and tight junctions in the neuroepithelial cells. We find that these proteins are distributed irregularly at the neural plate stage and become polarised apically in the cell membrane only during the keel/rod stage. The stereotypic orientation of mitoses is perturbed only weakly upon loss of function of the cell junction components ASIP and aPKClambda, suggesting that mitotic orientation depends in part on the integrity of cell junctions and the polarity of the epithelium as a whole. However, the 90degrees rotation of the spindle does not require perfectly polarised cell junctions between the neuroepithelial cells.