Hypothalamic neuropeptides, not leptin sensitivity, contributes to the hyperphagia in lactating Brandt's voles, Lasiopodomys brandtii
Hypothalamic neuropeptides, not leptin sensitivity, contributes to the hyperphagia in lactating Brandt's voles, Lasiopodomys brandtii
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DOI：
10.1242/jeb.054056
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发表时间：
2011-07
影响因子：
2.8
通讯作者：
J. Cui;G.‐B. Tang;Dehua Wang
中科院分区：
文献类型：
--
作者：
J. Cui;G.‐B. Tang;Dehua Wang
妊娠期和哺乳期都与暴食症有关，在布氏田鼠（Lasiopodomys brandtii）中，妊娠期循环瘦素水平升高，但在哺乳期下降。以前的研究结果表明，瘦素敏感性受损有助于在怀孕期间的食欲过盛。本研究旨在探讨布氏田鼠哺乳期摄食过多是否与循环瘦素水平和/或下丘脑瘦素敏感性降低有关。在动情间期、第5天、第6天、第7天、第8天检测血清瘦素水平以及长型瘦素受体（Ob-Rb）、细胞因子信号传导抑制因子-3（SOCS-3）、神经肽Y（NPY）、刺鼠相关蛋白（AgRP）、前阿黑皮素（POMC）以及下丘脑中可卡因和安非他明调节转录本（CART）的mRNA表达。布氏田鼠哺乳期第17天和断奶后第27天。与对照组相比，哺乳期大鼠下丘脑Ob-Rb和SOCS-3 mRNA的表达无明显变化。哺乳期女性血清瘦素水平显著低于非生育组。与对照组相比，哺乳期下丘脑NPY和AgRP mRNA表达显著增加，而POMC mRNA表达显著降低。下丘脑CART mRNA表达无明显变化。泌乳期摄食量与NPY和AgRP mRNA表达呈正相关，与POMC mRNA表达呈负相关。这些数据表明，在哺乳期的暴食与低瘦素水平，但不受损的瘦素敏感性，下丘脑神经肽NPY，AgRP和POMC参与介导的作用，瘦素在哺乳期布氏田鼠的食物摄入调节。
SUMMARY Both pregnancy and lactation are associated with hyperphagia, and circulating leptin levels are elevated during pregnancy but decreased during lactation in Brandt's voles, Lasiopodomys brandtii. Previous findings suggest that impaired leptin sensitivity contributes to hyperphagia during pregnancy. The present study aimed to examine whether the decreased circulating leptin level and/or hypothalamic leptin sensitivity contributed to the hyperphagia during lactation in Brandt's voles. The serum leptin level and mRNA expression of the long form of the leptin receptor (Ob-Rb), suppressor-of-cytokine-signalling-3 (SOCS-3), neuropeptide Y (NPY), agouti-related protein (AgRP), pro-opiomelanocortin (POMC) and cocaine- and amphetamine-regulated transcript (CART) in the hypothalamus were examined on dioestrous, day 5, day 17 of lactation and day 27 (1 week after weaning) in Brandt's voles. Compared with controls, hypothalamic Ob-Rb and SOCS-3 mRNA expression was not significantly changed during lactation. The serum leptin level was significantly lower in lactating females than in the non-reproductive group. Hypothalamic NPY and AgRP mRNA expression significantly increased whereas POMC mRNA expression was significantly decreased during lactation compared with controls. However, there were no significant changes in hypothalamic CART mRNA expression. Food intake was positively correlated with NPY and AgRP mRNA expression but negatively correlated with POMC mRNA expression during lactation. These data suggest that hyperphagia during lactation was associated with low leptin levels, but not impaired leptin sensitivity, and that the hypothalamic neuropeptides NPY, AgRP and POMC are involved in mediating the role of leptin in food intake regulation in lactating Brandt's voles.