Developmentally regulated Arabidopsis thaliana susceptibility to tomato spotted wilt virus infection
Developmentally regulated Arabidopsis thaliana susceptibility to tomato spotted wilt virus infection
复制标题
发育调控拟南芥对番茄斑萎病毒感染的易感性
DOI：
10.1111/mpp.12944
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发表时间：
2020-05-22
影响因子：
4.9
通讯作者：
Tao, Xiaorong
中科院分区：
文献类型：
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作者：
Huang, Ying;Hong, Hao;Tao, Xiaorong
摘要 番茄斑萎病毒（TSWV）是最具破坏性的植物病毒之一，经常在全球范围内造成严重的农作物损失。一般来说，成熟的植物对病原体的抵抗力更强，称为成体植物抗性。在这项研究中，我们证明了一种涉及拟南芥对 TSWV 的发育调节易感性的新现象。我们发现，随着植物成熟，拟南芥植物变得更容易受到 TSWV 的影响。大多数 3 周龄的拟南芥幼苗并未感染 TSWV。 4、5 和 6 周龄拟南芥中 TSWV 的感染率分别从 9%、21% 和 25% 增加到 7 至 8 周龄拟南芥植物中的 100%。 TSWV 的不同分离株和不同的拓扑病毒在幼年拟南芥中的感染率较低，但在成熟植物中的感染率较高。当拟南芥 dcl2/3/4 或 rdr1/2/6 突变体植物接种 TSWV 时，获得了与野生型拟南芥植物观察到的相似结果。细胞间运动测定表明，与 4 周龄拟南芥叶片相比，8 周龄拟南芥叶片中 TSWV NSm:GFP 融合体的细胞间运动效率显着更高。此外，在 TSWV 感染期间，与 4 周龄拟南芥叶片相比，在大分子细胞间运输中起关键作用的果胶甲酯酶和 β-1,3-葡聚糖酶的表达水平在 8 周龄拟南芥叶片中显着上调。迄今为止，这种成熟植物对病原体感染的易感性很少被研究。因此，这里提出的研究结果应该增进我们对发育调节的成熟宿主对植物病毒感染的易感性的认识。
Abstract Tomato spotted wilt virus (TSWV) is one of the most devastating plant viruses and often causes severe crop losses worldwide. Generally, mature plants become more resistant to pathogens, known as adult plant resistance. In this study, we demonstrated a new phenomenon involving developmentally regulated susceptibility of Arabidopsis thaliana to TSWV. We found that Arabidopsis plants become more susceptible to TSWV as plants mature. Most young 3‐week‐old Arabidopsis were not infected by TSWV. Infection of TSWV in 4‐, 5‐, and 6‐week‐old Arabidopsis increased from 9%, 21%, and 25%, respectively, to 100% in 7‐ to 8‐week‐old Arabidopsis plants. Different isolates of TSWV and different tospoviruses show a low rate of infection in young Arabidopsis but a high rate in mature plants. When Arabidopsis dcl2/3/4 or rdr1/2/6 mutant plants were inoculated with TSWV, similar results as observed for the wild‐type Arabidopsis plants were obtained. A cell‐to‐cell movement assay showed that the intercellular movement efficiency of TSWV NSm:GFP fusion was significantly higher in 8‐week‐old Arabidopsis leaves compared with 4‐week‐old Arabidopsis leaves. Moreover, the expression levels of pectin methylesterase and β‐1,3‐glucanase, which play critical roles in macromolecule cell‐to‐cell trafficking, were significantly up‐regulated in 8‐week‐old Arabidopsis leaves compared with 4‐week‐old Arabidopsis leaves during TSWV infection. To date, this mature plant susceptibility to pathogen infections has rarely been investigated. Thus, the findings presented here should advance our knowledge on the developmentally regulated mature host susceptibility to plant virus infection.