Prevalence of childhood wheeze and modified DNA methylation at 7 years of age according to maternal folate levels during pregnancy in the Hokkaido Study.
Prevalence of childhood wheeze and modified DNA methylation at 7 years of age according to maternal folate levels during pregnancy in the Hokkaido Study.
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北海道研究根据怀孕期间母亲的叶酸水平，得出儿童喘息和 7 岁时 DNA 甲基化修饰的患病率。
DOI：
10.1111/pai.13425
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发表时间：
2020
影响因子：
4.4
通讯作者：
Kishi Reiko
中科院分区：
文献类型：
--
作者：
Miyashita Chihiro;Araki Atsuko;Miura Ryu;Ait Bamai Yu;Kobayashi Sumitaka;Itoh Sachiko;Ito Kumiko;Tsai Meng‐Shan;Kishi Reiko
背景孕期服用大剂量叶酸可能会增加儿童时期哮喘、喘息和呼吸系统疾病的风险。叶酸可以通过包括DNA甲基化在内的表观遗传分化来改善后代的炎症和免疫易感性。这项研究评估了母亲孕期叶酸水平与儿童喘息之间的关系；此外，该研究还评估了母亲叶酸修饰的DNA甲基化是否与哮喘有关。方法本研究的参与者是6651对母子，他们拥有完整的特征数据，并在孩子1、2、4和7岁时至少完成了儿童哮喘和过敏国际调查问卷中的一项。结果化学发光免疫法测定母亲血清中甲基化水平的中位数为16.76nmol/L。我们发现，根据母亲的叶酸水平，2岁时儿童喘息的调整后优势比显著增加，而叶酸水平最低的四分位数(优势比[95%可信区间]=最高；1.27[1.03，1.56]和第二，1.27[1.05，1.55])；然而，在1岁、4岁和7岁时没有观察到变化。在病例对照研究中，未观察到母体叶酸水平与DNA甲基化的关系。结论我们的结果表明，母体叶酸不影响学龄儿童持续的喘息，也不影响7岁时Gasdermin B、类粘蛋白3和Ikaros家族锌指3的DNA甲基化。
BackgroundA high dose of folic acid during pregnancy may increase the risk of asthma, wheezing, and respiratory disease in childhood. Folate acid can modify inflammation and immune susceptibility of offspring with some epigenetic differentiation, including DNA methylation. This study evaluated associations between maternal folate levels during pregnancy and childhood wheezing; furthermore, the study assessed whether maternal folate‐modified DNA methylation is related to asthma.MethodsParticipants in the current study were 6651 mother‐child pairs who had complete data on characteristics and who had completed at least one of the International Study of Asthma and Allergies in Childhood questionnaires when the child was 1, 2, 4, and 7 years of age. Moreover, a case‐control study to assess DNA methylation at 7 years of age was conducted among 136 children who experienced wheezing and a control group of 139 children with no history of allergies.ResultsThe median of maternal serum was 16.76 nmol/L, assayed by chemiluminescent immunoassay. We found significantly increased adjusted odds ratios of childhood wheezing at 2 years age according to maternal folate levels, compared with the lowest folate quartile (odds ratio [95% confidence interval] = highest; 1.27 [1.03, 1.56], and second, 1.27 [1.05, 1.55]); however, no changes were observed at 1, 4, and 7 years of age. In a case‐control study, no association of maternal folate levels with DNA methylation was observed.ConclusionOur results suggest that maternal folate did not affect persistent wheezing in school‐aged children, or DNA methylation of gasdermin B, orosomucoid‐like 3, and Ikaros family zinc finger 3 at 7 years of age.