Ratiometric NIR cell membrane-targeted probe for monitoring cell membrane polarity and tumor application
Ratiometric NIR cell membrane-targeted probe for monitoring cell membrane polarity and tumor application
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DOI：
10.1016/j.snb.2024.135331
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发表时间：
2024-04
期刊：
影响因子：
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通讯作者：
Yongsheng Ma;Zhen-Qing Yu;Mengping Wang;Yuning Guan;Biyan Ding;Yan Zhang;Yanfeng Lv;Xiaofeng Yan
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文献类型：
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作者：
Yongsheng Ma;Zhen-Qing Yu;Mengping Wang;Yuning Guan;Biyan Ding;Yan Zhang;Yanfeng Lv;Xiaofeng Yan
细胞膜极性的研究对于确定细胞生理状态和相关疾病的诊断和治疗至关重要。在此，设计并合成了一种比率近红外细胞膜靶向荧光探针Cy-C12-MpN，用于评估细胞膜极性，该探针具有由十二烷基单元和季铵单元组成的两亲结构。随着溶液极性的变化，其发射波长和荧光强度发生变化。它可以使用两个峰值的比率信号来报告其环境中的极性差异。探头内部系统的自整定保证了测试结果的可靠性，可以是半定量的检测方法。事实上，在体外极性测试中，Cy-C12-MpN 报告的比率信号在极性方面具有最佳线性范围。细胞成像显示了Cy-C12-MpN优异的细胞膜靶向能力，它可以在30秒内快速靶向细胞膜，并在1小时后仍能很好地锚定在细胞膜上。此外，Cy-C12-MpN显示细胞癌变后细胞膜极性降低，首次观察到多不饱和脂肪酸刺激稳态膜反应引起的细胞膜极性变化。体内成像证明了Cy-C12-MpN区分肿瘤细胞和正常细胞的能力，并进一步证明肿瘤细胞具有较低的细胞膜极性。综上所述，探针Cy-C12-MpN的比率检测的准确性及其优异的细胞膜靶向能力揭示了可靠的结果，可以为癌症研究提供非常有用的见解。
The investigation of cell membrane polarity is critical to determining cell physiological states and related disease for diagnosis and treatment. Herein, a ratiometric NIR cell membrane-targeted fluorescent probe,Cy-C12-MpN, was designed and synthesized to assess cell membrane polarity, which has an amphiphilic structure consisting of a dodecyl unit and a quaternary ammonium unit. It exhibited changes in emission wavelength and fluorescence intensity with the changes in polarity of the solution. It can use the ratio signal of the two peaks to report the polarity difference in its environment. The self-tuning of the probe internal system ensures the reliability of the test results and can be a semi-quantitative detection method. Indeed, in vitro polarity test, the ratio signal reported byCy-C12-MpNhad an optimal linear range with respect to polarity. Cell imaging illustrated the excellent cell membrane-targeted ability ofCy-C12-MpN, as it can rapidly target the cell membrane within 30 s and still anchor at it well after 1 hr. Moreover,Cy-C12-MpNshowed that cell membrane polarity decreases after cells become cancerous, and observed the changes in cell membrane polarity caused by homeostatic membrane response stimulated by polyunsaturated fatty acids firstly. Imaging in vivo demonstrated the ability ofCy-C12-MpNto distinguish between tumor cells and normal cells, and further demonstrated that tumor cells have lower cell membrane polarity. In summary, the accuracy of the ratio detection of the probeCy-C12-MpNand its excellent cell membrane targeting ability reveal reliable results that can provide very useful insights for cancer research.