Chemical methods and advanced sequencing technologies for deciphering mRNA modifications
Chemical methods and advanced sequencing technologies for deciphering mRNA modifications
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用于破译 mRNA 修饰的化学方法和先进测序技术
DOI：
10.1039/d1cs00920f
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发表时间：
2021
影响因子：
46.2
通讯作者：
Zhou Xiang
中科院分区：
文献类型：
--
作者：
Wang Yafen;Zhang Xiong;Liu Hui;Zhou Xiang
RNA修饰与其他表观遗传修饰如DNA修饰和组蛋白修饰一样，是基因表达转录后调控领域的新兴参与者。目前已鉴定出的RNA修饰类型超过160种，它们广泛分布于不同类型的RNA中。最近，研究人员越来越多地使用先进的技术来研究修饰的核酸，以阐明其生物学功能，并扩大对表观遗传学中心规律的理解。在本教程综述中，我们全面概述了当前用于解码RNA修饰的先进技术，突出了现有方法中的一些瓶颈以及可能导致创新的新机会。通过这篇综述，我们希望为化学和生物学学生和研究人员提供解决一些具有挑战性的问题的想法，例如如何同时检测同一系统中的多种类型的修饰。此外，一些低覆盖的修饰，可能作为“候选人”在重要的转录过程需要进一步探索。这些新方法有可能为理解RNA修饰的生物学功能的细微复杂性奠定基础。
RNA modification, like other epigenetic modifications such as DNA modification and histone modification, is an emerging player in the field of the posttranscriptional regulation of gene expression. More than 160 kinds of RNA modifications have been identified, and they are widely distributed in different types of RNA. Recently, researchers have increasingly used advanced technologies to study modified nucleic acids in order to elucidate their biological functions and expand the understanding of the central laws of epigenetics. In this tutorial review, we comprehensively outline current advanced techniques for decoding RNA modifications, highlighting some of the bottlenecks in existing approaches as well as new opportunities that may lead to innovations. With this review, we expect to provide chemistry and biology students and researchers with ideas for solving some challenging problems, such as how to simultaneously detect multiple types of modifications within the same system. Moreover, some low-coverage modifications that may act as ‘candidates’ in important transcriptional processes need to be further explored. These novel approaches have the potential to lay a foundation for understanding the nuanced complexities of the biological functions of RNA modification.