Sustained release of vascular endothelial growth factor from mineralized poly(lactide-co-glycolide) scaffolds for tissue engineering
Sustained release of vascular endothelial growth factor from mineralized poly(lactide-co-glycolide) scaffolds for tissue engineering
复制标题
DOI：
10.1016/s0142-9612(00)00120-4
复制
发表时间：
2000-12-01
期刊：
影响因子：
14
通讯作者：
Mooney, DJ
中科院分区：
文献类型：
--
作者：
Murphy, WL;Peters, MC;Mooney, DJ
骨组织工程的策略主要集中在：(1)使用支架进行成骨细胞移植或作为引导骨再生的导电基质；或(2)从这些支架材料中释放诱导性生物活性因子。本研究描述了一种在骨组织工程的骨传导支架上添加诱导性成分的方法。我们报道了生物活性血管内皮生长因子(VEGF)从矿化的、多孔的、可降解的聚合物支架中释放出来。通过在气体发泡/颗粒浸出过程中加入生长因子，制备了聚丙交酯-乙交酯共聚物85：15的三维多孔支架。然后通过在模拟体液中孵育使支架矿化。通过质量增加测量和支架内磷酸盐含量的定量测定，证实了在多孔支架的内孔表面上生长了骨样矿物膜。在15天内跟踪I-125标记的血管内皮生长因子的释放，以确定矿化支架的释放动力学。矿化支架的缓释效果良好，矿物膜的生长对支架的释药动力学影响不大。矿化处理后12天，矿化和非矿化支架释放的血管内皮生长因子活性均在70%以上，矿物生长对支架总孔隙率影响不大。(C)2000爱思唯尔科学有限公司。保留所有权利。
Strategies to engineer bone tissue have focused on either: (1) the use of scaffolds for osteogenic cell transplantation or as conductive substrates for guided bone regeneration; or(2) release of inductive bioactive factors from these scaffold materials. This study describes an approach to add an inductive component to an osteoconductive scaffold for bone tissue engineering. We report the release of bioactive Vascular endothelial growth factor (VEGF) from a mineralized, porous, degradable polymer scaffold. Three dimensional, porous scaffolds of the copolymer 85:15 poly(lactide-co-glycolide) were fabricated by including the growth factor into a gas foaming/particulate leaching process. The scaffold was then mineralized via incubation in a simulated body fluid. Growth of a bone-like mineral film on the inner pore surfaces of the porous scaffold is confirmed by mass increase measurements and quantification of phosphate content within scaffolds. Release of I-125-labeled VEGF was tracked over a 15 day period to determine release kinetics from the mineralized scaffolds. Sustained release from the mineralized scaffolds was achieved, and growth of the mineral film had only a minor effect on the release kinetics from the scaffolds. The VEGF released from the mineralized and non-mineralized scaffolds was over 70% active for up to 12 days following mineralization treatment, and the growth of mineral had little effect on total scaffold porosity. (C) 2000 Elsevier Science Ltd. All rights reserved.