RAPID GENOTYPING TECHNIQUES for IDENTIFICATION of SPECIES and STOCK IDENTITY in FRESH, FROZEN, COOKED and CANNED WHALE PRODUCTS
RAPID GENOTYPING TECHNIQUES for IDENTIFICATION of SPECIES and STOCK IDENTITY in FRESH, FROZEN, COOKED and CANNED WHALE PRODUCTS
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用于鉴定新鲜、冷冻、煮熟和罐装鲸鱼产品中的物种和库存身份的快速基因分型技术
DOI：
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
S. Palumbi
中科院分区：
文献类型：
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作者：
F. Cipriano;S. Palumbi
用于鲸鱼产品鉴定的 DNA 测序方法受到限制，因为此类方法昂贵且耗时。因此，考虑到对当前和新兴鲸鱼产品市场进行基因调查的需要，每次调查中可以处理和分析的样本数量太少。利用高通量基因分型技术，我们正在开发更快速、更具成本效益的检测程序来识别鲸类产品。我们设计了用于此类技术的分子探针，并已开始测试该方法用于商业鲸鱼市场的大规模调查。这种鲸鱼产品识别技术将使国际计划能够准确监控商业鲸鱼产品的身份，并允许未来商业捕鲸的任何增加对受威胁物种和种群的影响最小化。我们使用来自日本商业鲸鱼市场（N=130）的新（1999）调查的产品以及之前调查中收集的其他物种的产品测试了分子探针。 1999年调查中发现的受保护的大鲸鱼包括两种来自不同座头鲸的产品、两种来自抹香鲸的产品和一种来自布氏鲸的产品。 1999 年调查中确定的鲸鱼产品子样本也接受了污染物分析，因此与商业食品相关的污染物负荷可能与原产地物种和广告标识相关。 18 种产品在广告中声称源自一种物种，但同时含有来自另一种物种的产品，而 19 种未识别或错误识别的“鲸鱼”产品则源自齿鲸。 11 种产品正确地宣传为齿鲸，7 种未广告或错误宣传的含齿鲸产品进行了污染物分析，其中至少含有一种达到人类消费者关注水平的污染物类型。
DNA sequencing methods used for whale product identification are limited because such methods are expensive and time consuming. As a result, the number of samples that can be processed and analyzed in each survey is too small given the need for genetic surveys of current and emerging whale product markets. Using high-throughput genetic typing technology, we are developing more rapid and costefficient assay procedures to identify cetacean products. We designed molecular probes for use in one such technique and have begun testing this method for use in large-scale surveys of commercial whale markets. Such techniques for whale product identification will allow international programs to accurately monitor the identity of commercial whale products, and allow any future increase in commercial whaling to be accomplished with minimal impact on threatened species and populations. We tested molecular probes with products from a new (1999) survey of Japanese commercial whale markets (N=130) and with products from additional species collected in previous surveys. Protected great whales detected in the 1999 survey included two products derived from different humpback whales, two products derived from sperm whales, and one product derived from a Bryde’s whale. Subsamples of whale products identified in the 1999 survey were also subjected to pollutant analysis, so that pollutant loads associated with commercial food products could be related to species of origin and advertised identity. Eighteen products were advertised as derived from one species while containing products from another, while nineteen unidentified or misidentified“whale” products were derived from odontocetes. Eleven products correctly advertised as odontocete and seven of the un- or mis-advertised odontocete-containing products which were analyzed for pollutants contained at least one pollutant type at a level of concern for human consumers.
影响因子：
4.4
作者：
SHEFFIELD, VC;BECK, JS;STONE, EM
通讯作者：
STONE, EM
DOI：
10.1073/pnas.95.4.1681
发表时间：
1998-02-17
影响因子：
11.1
作者：
Körkkö, J;Annunen, S;Ala-Kokko, L
通讯作者：
Ala-Kokko, L
DOI：
10.1016/s1050-3862(98)00018-7
发表时间：
1999-02-01
期刊：
GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING
影响因子：
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作者：
Marras, SAE;Kramer, FR;Tyagi, S
通讯作者：
Tyagi, S