The Genetic Basis of Scale-Loss Phenotype in the Rapid Radiation of Takifugu Fishes
The Genetic Basis of Scale-Loss Phenotype in the Rapid Radiation of Takifugu Fishes
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DOI：
10.3390/genes10121027
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发表时间：
2019-12-01
期刊：
影响因子：
3.5
通讯作者：
Kikuchi, Kiyoshi
中科院分区：
文献类型：
--
作者：
Kim, Dong In;Kai, Wataru;Kikuchi, Kiyoshi
快速辐射与表型的分歧和收敛提供了一个机会，研究进化的遗传机制。在这里，我们调查属东方相对最近经历了爆炸性辐射，并包含一个子集的密切相关的物种与规模损失表型。利用发育过程中的观察和遗传作图方法，我们发现两种东方鲀的鳞片损失表型，T。pardalis Temminck & Schlegel和T. snyderi Abe，主要由重叠基因组片段（QTL）控制。寻找潜在的鳞片损失表型的候选基因显示，QTL区域不包含已知的基因负责鳞片损失表型在其他鱼类的进化。这些结果表明，在两个东方鲀的鳞片损失表型的基因可能是相同的，但在东方鲀和远亲鱼的相似表型的基因是不一样的。同时，在QTL区域中鉴定了Fgfrl 1，一种预测在已知调节骨/鳞片发育的途径中起作用的基因。由于Fgfr 1a 1，Fgf信号通路的另一个memebr，已牵连在鳞片损失/鳞片形状的鱼类远亲东方鲀，我们的研究结果表明，收敛的鳞片损失表型可能受到限制的信号模块与保守的作用，在规模发展。
Rapid radiation associated with phenotypic divergence and convergence provides an opportunity to study the genetic mechanisms of evolution. Here we investigate the genus Takifugu that has undergone explosive radiation relatively recently and contains a subset of closely-related species with a scale-loss phenotype. By using observations during development and genetic mapping approaches, we show that the scale-loss phenotype of two Takifugu species, T. pardalis Temminck & Schlegel and T. snyderi Abe, is largely controlled by an overlapping genomic segment (QTL). A search for candidate genes underlying the scale-loss phenotype revealed that the QTL region contains no known genes responsible for the evolution of scale-loss phenotype in other fishes. These results suggest that the genes used for the scale-loss phenotypes in the two Takifugu are likely the same, but the genes used for the similar phenotype in Takifugu and distantly related fishes are not the same. Meanwhile, Fgfrl1, a gene predicted to function in a pathway known to regulate bone/scale development was identified in the QTL region. Since Fgfr1a1, another memebr of the Fgf signaling pathway, has been implicated in scale loss/scale shape in fish distantly related to Takifugu, our results suggest that the convergence of the scale-loss phenotype may be constrained by signaling modules with conserved roles in scale development.