Effects of southern rice black-streaked dwarf virus on the development and fecundity of its vector, Sogatella furcifera.
Effects of southern rice black-streaked dwarf virus on the development and fecundity of its vector, Sogatella furcifera.
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DOI：
10.1186/1743-422x-10-145
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发表时间：
2013-05-12
期刊：
影响因子：
4.8
通讯作者：
Zhou G
中科院分区：
文献类型：
--
作者：
Tu Z;Ling B;Xu D;Zhang M;Zhou G
南方水稻黑条矮缩病毒（SRBSDV）威胁着中国和越南的水稻生产。该病毒是由迁移的白背飞虱（白背飞虱，白背飞虱）在循环，繁殖和持久的方式。持续传播的植物病毒可通过感染载体本身和/或间接改变宿主植物来直接影响其载体的发育和繁殖力。本研究评估了SRBSDV对白背飞虱表现的直接和间接影响，以更好地了解病毒-载体-宿主植物的关系，其对载体的生物学参数的影响。建立了三个实验种群。将感染SRBSDV的水稻幼苗作为一龄幼虫饲养48 h后，用RT-PCR检测感染状态。对照组食用健康大米。将每种昆虫单独转移到在20°C、25°C或28°C下在玻璃管中生长的健康水稻植物中。在不同温度下测定了各种群若虫历期、存活率、成虫性比、长翅目比例、寿命和产卵量等生命参数。生命参数数据表明，SRBSDV和感染水稻植株的白背飞虱的不利影响，影响是温度依赖性。与对照种群相比，带病毒种群表现出显著的变化，包括若虫期延长和20°C下存活率降低，而不带病毒种群与对照相比，在20°C下存活率较高，在28°C下存活率较低。与对照相比，两个种群在25°C下的成虫寿命显著缩短，在28°C下的产卵量显著降低。SRBSDV感染和取食受感染的水稻植株都影响了媒介的表现。虽然较长的若虫期有利于病毒的获得和传播，并可能增加水稻的感染率，但总体而言，媒介和寄主植物的SRBSDV感染不利于白背飞虱种群的扩大。
Southern rice black-streaked dwarf virus (SRBSDV) threatens rice production in China and Vietnam. The virus is vectored by the migrating white-backed planthopper (WBPH, Sogatella furcifera) in a circulative, propagative, and persistent manner. A persistently-transmitted plant virus might affect its vector’s development and fecundity directly by infecting the vector itself and/or indirectly altering the host plant. This study evaluated the direct and indirect effects of SRBSDV on WBPH performance to better understand the virus–vector–host plant relationship in terms of its effects on the biological parameters of the vector. Three experimental WBPH populations were established. Viruliferous and non-viruliferous populations were fed on SRBSDV-infected rice seedlings for 48 h as first-instar nymphs; infection status was confirmed by RT–PCR after they died. The control population was fed on healthy rice. Each insect was individually transferred to a healthy rice plant grown in a glass tube at 20°C, 25°C, or 28°C. Life parameters, including nymphal duration, survival rate, adult sex ratio, macropterous proportion, longevity, and oviposition amounts, of each population were measured at each temperature. The life parameter data indicated that SRBSDV and infected rice plants adversely influenced WBPH; the effects were temperature dependent. Compared with the control population, viruliferous populations showed significant changes, including prolonged nymphal stages and reduced survival rates at 20°C, while the non-viruliferous population had higher survival rates at 20°C and lower rates at 28°C compared with the control. Both populations had significantly shorter adult life spans at 25°C and lower oviposition amounts at 28°C relative to the control. Both SRBSDV-infection and feeding on infected rice plants affected vector performance. Although a longer nymphal period benefits viral acquisition and transmission by nymphs and might increase rice infection rate, in general, SRBSDV infection of the vectors and host plants was unfavorable to WBPH population expansion.