Determination of the distribution of sodium and potassium ion activated adenosinetriphosphatase among the various oligomers formed in solutions of nonionic detergents.
Determination of the distribution of sodium and potassium ion activated adenosinetriphosphatase among the various oligomers formed in solutions of nonionic detergents.
复制标题
测定非离子洗涤剂溶液中形成的各种低聚物中钠离子和钾离子激活的腺苷三磷酸酶的分布。
DOI：
10.1021/bi00540a014
复制
发表时间：
1982
期刊：
影响因子：
2.9
通讯作者：
Craig,WS
中科院分区：
文献类型：
--
作者：
Craig,WS
William S. Craig* 摘要：通过使用均匀的非离子洗涤剂八乙二醇十二烷基醚溶液处理，钠离子和钾离子激活的三磷酸腺苷 [(Na++ K+)-ATPase] 可以从膜结合状态分散，并稳定保留显示 (Na++ K+)-ATPase 活性的能力。然而，在游离去垢剂浓度高于临界胶束浓度的溶液中，分散的酶不能转换。用交联剂戊二醛处理该酶的溶液导致α-和/3-多肽的定量共价偶联。在十二烷基钠上观察时形成的各种共价产物
William S. Craig* abstract: Sodium and potassium ion activated adenosine-triphosphatase [(Na++ K+)-ATPase] can be dispersed from the membrane-bound state, with the stable retention of the capacity to display (Na++ K+)-ATPase activity, by treatment with solutions of a homogeneous, nonionic detergent, octa-ethylene glycol dodecyl ether. The dispersed enzyme is in-capable of turnover, however, in solutions where the free de-tergent concentration is above the critical micelle concentration. Treatment of solutions of this enzyme with the cross-linking reagent glutaraldehyde results in the quantitative, covalent coupling of the a-and/3-polypeptides. The various covalent products formed, when visualized on sodium dodecyl