Diethylstilbestrol induces fish oocyte maturation
Diethylstilbestrol induces fish oocyte maturation
复制标题
DOI：
10.1073/pnas.0400072101
复制
发表时间：
2004-03-09
影响因子：
11.1
通讯作者：
Nagahama, Y
中科院分区：
文献类型：
--
作者：
Tokumoto, T;Tokumoto, M;Nagahama, Y
内分泌干扰化学物质己烯雌酚（DES），一种非甾体雌激素，触发鱼类卵母细胞成熟。DES诱导的形态学（生发囊泡破裂变化的时间过程）和细胞内分子事件（细胞周期蛋白B的从头合成）与天然成熟诱导激素17 α，20 β-二羟基-4-异戊烯-3-酮（17，20 β-DHP）诱导的形态学和细胞内分子事件没有区别。DES对17，20 β-DHP诱导的卵母细胞成熟有协同作用。17，20 β-DHP和DES诱导的卵母细胞成熟均被成熟诱导激素受体抗体抑制。DES的行动的结构要求进行了讨论的基础上与DES类似物获得的结果。
An endocrine-disrupting chemical, diethylstilbestrol (DES), a nonsteroidal estrogen, triggers oocyte maturation in fish. The morphology (the time course of the change in germinal vesicle breakdown) and an intracellular molecular event (the de novo synthesis of cyclin B) induced by DES were indistinguishable from those induced by a natural maturation-inducing hormone, 17alpha,20beta-dihydroxy-4-pregnen-3-one (17,20beta-DHP). A synergistic action of DES on 17,20beta-DHP-induced oocyte maturation was observed. Both 17,20beta-DHP- and DES-induced oocyte maturation was inhibited by an antibody against the maturation-inducing hormone receptor. The structural requirement for the action of DES is discussed based on results obtained with DES analogs.