Matrix protein gene expression in intervertebral disc cells subjected to altered osmolarity
Matrix protein gene expression in intervertebral disc cells subjected to altered osmolarity
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DOI：
10.1016/s0006-291x(02)00314-5
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发表时间：
2002-05-10
影响因子：
3.1
通讯作者：
Setton, LA
中科院分区：
文献类型：
--
作者：
Chen, J;Baer, AE;Setton, LA
椎间盘的生理负荷可能导致常驻细胞所经历的渗透压的变化。在这项研究中，细胞外基质和细胞骨架蛋白的基因表达水平在低渗透(255mOsm)和高渗透(450mOsm)条件下与等渗(293mOsm)条件下的盘状细胞中进行了定量比较。不同区域的细胞间渗透压有显著差异，分别对应于移行区和髓核。在低渗条件下，胶原蛋白多糖和II型胶原的基因表达在过渡区上调。但不在髓核细胞中。在低渗或高渗介质中孵育后，在过渡区细胞中，小蛋白多糖、大聚糖和核心蛋白聚糖的基因表达上调，但不包括鲁米肯。在低渗或高渗条件下，相同的基因在髓核细胞中下调。椎间盘细胞对渗透压改变的反应差异可能与其不同的细胞骨架结构或胚胎学起源有关。(C)2002年埃尔塞维尔科学公司(美国)。版权所有。
Physiologic loading of the intervertebral disc may lead to changes in the osmotic pressure experienced by the resident cells. In this study, changes in gene expression levels for extracellular matrix and cytoskeletal proteins were quantified in disc cells subjected to hypo-osmotic (255 mOsm) or hyper-osmotic conditions (450 mOsm), relative to iso-osmotic conditions (293 mOsm). Important differences were observed in osmolarity and between cells of different regions, corresponding to the transition zone and nucleus pulposus. Under hypo-osmotic conditions, gene expressions for aggrecan and type II collagen were up-regulated in the transition zone. but not in the nucleus pulposus cells. Genes for the small proteoglycans, biglycan, and decorin, but not lumican, were up-regulated in transition zone cells following incubation in either hypo- or hyper-osmotic media. The same genes were down-regulated in nucleus pulposus cells under either hypo- or hyper-osmotic conditions. Differences in the response to altered osmolarity between cells of the intervertebral disc may relate to their different cytoskeletal structures or embryological origins. (C) 2002 Elsevier Science (USA). All rights reserved.