Axonal neurofilament-H immunolabeling in the rabbit retina.
Axonal neurofilament-H immunolabeling in the rabbit retina.
复制标题
兔视网膜中的轴突神经丝-H 免疫标记。
DOI：
10.1002/cne.10392
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发表时间：
2002
期刊：
影响因子：
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通讯作者：
Bloomfield,StewartA
中科院分区：
文献类型：
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作者：
Völgyi,Béla;Bloomfield,StewartA
使用针对轴突神经丝-H（NF-H）的多磷酸化表位的单克隆抗体标记兔视网膜中的轴突样纤维。NF-H-免疫阳性纤维见于外丛状层（OPL）、内丛状层（IPL）和光纤层（OFL）。标记突起的形态特征表明OPL中的突起为水平细胞轴突，OFL中的轴突终末和纤维为神经节细胞轴突。OPL中的NF-H-阳性分布形成了钙结合蛋白标记的水平细胞突起的子集。在IPL中，NF‐H‐免疫反应性分布在所有水平，但最常在中间层2-4中检测到。偶尔，我们观察到NF-H-免疫反应过程从IPL出现并进入GCL或内核层（INL）。IPL中的标记纤维通常非常细，直径小于1 μm，并且当它们横向穿过视网膜时通常可以跟踪超过1 mm。细胞体从未被免疫血清标记。为了鉴定IPL中的NF-H-免疫阳性纤维，应用标准免疫细胞化学双标记技术，使用针对被认为由轴突承载无长突细胞表达的几种神经递质或调节剂的抗体。IPL中的NF-H-阳性过程与标记为酪氨酸羟化酶、生长抑素、P物质和NADPH黄递酶活性的过程相对应。然而，NF-H标记不与针对血管活性肠肽相关蛋白PHM 27的标记共定位。我们的研究结果表明，视网膜中假定的轴突具有在中枢神经系统的经典轴突中发现的多磷酸化NF-H蛋白。因此，这些结果支持的想法，某些亚型的无长突和水平细胞维持真正的轴突在哺乳动物视网膜。J. Comp.神经元453：269-279，2002.© 2002 Wiley利斯公司
A monoclonal antibody directed at the multiphosphorylated epitope of axonal neurofilament‐H (NF‐H) was used to label axon‐like fibers in the rabbit retina. NF‐H‐immunopositive fibers were found in the outer plexiform layer (OPL), inner plexiform layer (IPL), and optic fiber layer (OFL). The morphological characteristics of the labeled processes identified those in the OPL as horizontal cell axons and axon terminals and fibers in the OFL as axons of ganglion cells. The NF‐H‐positive profiles in the OPL formed a subset of horizontal cell processes labeled for calbindin. In the IPL, NF‐H‐immunoreactive profiles lay at all levels but were detected most often in the middle strata, 2–4. Occasionally, we observed NF‐H‐immuoreactive processes emerging from the IPL and entering either the GCL or the inner nuclear layer (INL). The labeled fibers in the IPL were typically very thin, less than 1 μm in diameter, and could often be followed for over 1 mm as they ran laterally across the retina. Cell bodies were never labeled by the immunoserum. To identify the NF‐H‐immunopositive fibers in the IPL, standard immunocytochemical double‐labeling techniques were applied, using antibodies directed against several neurotransmitters or modulators thought to be expressed by axon‐bearing amacrine cells. The NF‐H‐positive processes in the IPL were found to correspond to those labeled for tyrosine hydroxylase, somatostatin, substance P, and NADPH diaphorase activity. However, the NF‐H labels did not colocalize with those against the vasoactive intestinal peptide‐associated protein PHM27. Our results indicate that putative axons in the retina possess the multiphosphorylated NF‐H protein found within classic axons in the central nervous system. These results thus support the idea that certain subtypes of amacrine and horizontal cells maintain true axons in the mammalian retina. J. Comp. Neurol. 453:269–279, 2002. © 2002 Wiley‐Liss, Inc.