Protein kinase activity associated with the product of the yeast cell division cycle gene CDC28.
Protein kinase activity associated with the product of the yeast cell division cycle gene CDC28.
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DOI：
10.1073/pnas.82.12.4055
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发表时间：
1985-06
影响因子：
11.1
通讯作者：
Steven I. Reed;J. Hadwiger;Attila T. Lorincz
中科院分区：
文献类型：
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作者：
Steven I. Reed;J. Hadwiger;Attila T. Lorincz
针对由lacZ-CDC 28框内融合体编码的蛋白质产生的抗体显示从酵母细胞裂解物免疫沉淀CDC 28产物。多肽p36 CDC 28是表观Mr为36，000的磷蛋白。发现通过使用抗-CDC 28抗体从酵母细胞裂解物制备的免疫复合物具有蛋白激酶活性，如通过将标记从[γ-32 P]ATP转移到未知身份或功能的共沉淀的Mr 40，000蛋白质（p40）所测定的。当从几种不同的cdc 28温度敏感菌株中制备提取物时，这种活性是不存在的或不耐热的。蛋白激酶活性依赖于Zn ~（2+），并特异性地将磷酸转移到丝氨酸和苏氨酸残基。
Antibodies raised against the protein encoded by a lacZ-CDC28 in-frame fusion were shown to immunoprecipitate the CDC28 product from yeast cell lysates. The polypeptide p36CDC28 is a phosphoprotein of apparent Mr 36,000. Immune complexes prepared from yeast cell lysates by using anti-CDC28 antibody were found to possess a protein kinase activity, as determined by the transfer of label from [gamma-32P]ATP to a coprecipitated Mr 40,000 protein of unknown identity or function (p40). This activity was absent or thermolabile when extracts were prepared from several different cdc28 temperature-sensitive strains. The protein kinase activity was dependent on Zn2+ and transferred phosphate specifically to serine and threonine residues.