Paradoxical action of activin A on folliculogenesis in immature and adult mice.
Paradoxical action of activin A on folliculogenesis in immature and adult mice.
复制标题
激活素 A 对未成熟和成年小鼠卵泡发生的矛盾作用。
DOI：
--
复制
发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Y. Ibuki
中科院分区：
文献类型：
--
作者：
H. Yokota;Kiyohiko Yamada;Xiaowei Liu;J. Kobayashi;Y. Abe;Hideki Mizunuma;Y. Ibuki
激活素 A 是一种最初从卵泡液中分离出来的性腺蛋白，被认为是颗粒细胞分化的局部调节剂。然而，激活素 A 是否促进卵泡发生仍不清楚。本研究旨在阐明激活素 A 对体外卵泡培养系统中卵泡生长的影响。从 BDF1 杂交未成熟小鼠（11 天龄）和成年小鼠（8 周龄）中机械分离直径 100-120 μm 的腔前卵泡，然后在补充有激活素 A (100 ng/ml)、FSH (100 mIU/ml) 及其组合的无血清培养基中培养 4 天。每天测量卵泡直径，并通过 RIA 测定第 4 天释放的雌二醇和抑制素的量。当与激活素 A 或激活素 A 和 FSH 一起培养时，从未成熟小鼠收集的腔前卵泡显示出直径显着增加。单独使用 FSH 对未成熟小鼠的卵泡直径没有显着影响。相比之下，直径...
Activin A is a gonadal protein originally isolated from follicular fluid and is recognized as a local regulator of granulosa cell differentiation. Whether activin A promotes folliculogenesis, however, still remains unclarified. The present study was designed to elucidate the effect of activin A on follicular growth in in vitro follicle culture systems. Preantral follicles, 100–120 μm in diameter, were mechanically isolated from BDF1 hybrid immature mice (11 days old) and adult mice (8 weeks old), then cultured for 4 days in a serum-free medium supplemented with activin A (100 ng/ml), FSH (100 mIU/ml), and a combination of both. Follicular diameter was measured daily, and the amount of estradiol and inhibin released at day 4 was determined by RIA. Preantral follicles collected from immature mice showed a significant increase in diameter when cultured with activin A or both activin A and FSH. FSH alone showed no significant effect on the diameter of follicles from immature mice. In contrast, the diameter of...
DOI：
10.1530/jrf.0.0990403
发表时间：
1993-11
期刊：
Journal of reproduction and fertility
影响因子：
--
作者：
Xiaohong Wang;G. S. Greenwald
通讯作者：
Xiaohong Wang;G. S. Greenwald
激活素刺激培养的颗粒细胞中的抑制素分泌和信使 RNA 水平。
DOI：
10.1210/mend-3-10-1666
发表时间：
1989
期刊：
Molecular endocrinology (Baltimore, Md.)
影响因子：
--
作者：
LaPolt,PS;Soto,D;Su,JG;Campen,CA;Vaughan,J;Vale,W;Hsueh,AJ
通讯作者：
Hsueh,AJ