pH-switchable bacteria detection using zwitterionic fluorescent polymer.
pH-switchable bacteria detection using zwitterionic fluorescent polymer.
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DOI：
10.1016/j.bios.2016.12.013
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发表时间：
2017-04
影响因子：
12.6
通讯作者：
Khoerunnisa;Z. Mazrad;I. In;S. Park
中科院分区：
文献类型：
--
作者：
Khoerunnisa;Z. Mazrad;I. In;S. Park
构建了对pH变化具有高灵敏度的两性离子荧光聚合物，用于革兰氏阳性和革兰氏阴性病原菌的检测和成像。以单硼二吡咯甲烷（BODIPY）为疏水染料，溴乙烷为阳离子基团，合成了一种用于细菌结合的两性离子荧光聚合物（BOD/BE-PSM）检测探针。两性离子型荧光聚合物通过细菌表面上的阴离子基团和阳离子BOD/BE-PSM基团之间的离子络合物在1小时孵育后结合到细菌。这一发现表明，荧光开/关系统在固定的1 mg/mL聚合物浓度下通过两性离子荧光聚合物的亲水性和疏水性的变化来操作，这取决于pH（6.0、7.4或9.0）。该体系具有良好的稳定性，检测限为1 mg/mL。还观察到与疏水性BODIPY染料相互作用引起的淬灭，使得能够进行细菌检测，如荧光光谱和共聚焦显微镜图像所示。我们的研究结果表明，两性离子荧光聚合物可用于检测在较宽的pH值范围内的细菌。
A zwitterionic fluorescent polymer with high sensitivity to pH changes was constructed for the detection and imaging of both gram-positive and gram-negative pathogenic bacteria. A detection probe using the zwitterionic fluorescent polymer was synthesized with single boron dipyrromethane (BODIPY) as a hydrophobic dye and bromoethane as a cationic group for bacteria binding with conjugated poly(sulfobetaine methacrylate) (BOD/BE-PSM). The zwitterionic fluorescent polymer bound to bacteria through ionic complexes between anionic groups on the bacterial surface and cationic BOD/BE-PSM groups after 1 h incubation. This finding demonstrated that the fluorescence on/off system operated via changes in the hydrophilic and hydrophobic nature of the zwitterionic fluorescent polymer, depending on the pH (6.0, 7.4, or 9.0), at a fixed 1 mg/mL polymer concentration. The system showed good stability with a limit of detection of 1 mg/mL. Quenching caused by interactions with the hydrophobic BODIPY dye was also observed, enabling bacteria detection, as shown by fluorescence spectroscopy and confocal microscopy images. Our results indicated that the zwitterionic fluorescent polymer could be used to detect bacteria over a wide range of pH values.