DIFFERENTIAL ASSOCIATION OF TRANSFER-RNAS WITH THE GENOMES OF MURINE, FELINE AND PRIMATE RETROVIRUSES
DIFFERENTIAL ASSOCIATION OF TRANSFER-RNAS WITH THE GENOMES OF MURINE, FELINE AND PRIMATE RETROVIRUSES
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DOI：
10.1016/0005-2787(80)90139-2
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发表时间：
1980-01-01
期刊：
影响因子：
--
通讯作者：
POPP, RA
中科院分区：
文献类型：
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作者：
WATERS, LC;MULLIN, BC;POPP, RA
鉴定了与几种鼠、猫和灵长类逆转录病毒的基因组RNA结合的tRNA。通过逐步热解离70 S RNA将转移RNA分为松散结合的和紧密结合的。然后通过氨酰化对其进行鉴定和半定量。脯氨酸tRNA是几种鼠白血病病毒株、两种猫白血病病毒株以及灵长类动物病毒猿肉瘤、狒狒内源性和类人猿淋巴瘤中结合最牢固的tRNA。在猫异嗜性病毒RD-114中，tRNAGly富集在结合最紧密的组分中。在Mason-Pfizer猴病毒中，与鼠乳腺肿瘤病毒一样，tRNALys是与其基因组RNA结合最牢固的tRNA。除了最紧密相关的tRNA外，还发现一种或多种不同的tRNA与70 S RNA相关。(For为了方便起见，从所研究的任何逆转录病毒中分离的最大RNA复合物（50-70 S）被称为70 S RNA。这些tRNA可以与最紧密结合的tRNA区分开来，因为它们可以在较低温度下解离。它们以与紧密结合的tRNA相同的相对丰度出现。
The tRNA that are bound to the genomic RNA of several murine, feline and primate retroviruses were identified. Transfer RNA were divided into those loosely bound and those tightly bound by stepwise thermal dissociation of the 70 S RNA. They were then identified and semiquantitated by aminoacylation. Proline tRNA is the most tenaciously bound tRNA in several strains of murine leukemia virus, 2 strains of feline leukemia virus, and the primate viruses simian sarcoma, baboon endogenous and gibbon ape lymphoma. In the feline xenotropic virus, RD-114, tRNAGly is enriched in the most tightly bound fraction. In Mason-Pfizer monkey virus, as in the murine mammary tumor virus, tRNALys is the tRNA most tenaciously bound to its genomic RNA. Besides the most tightly associated tRNA, one or more different tRNA are found in relatively large amounts in association with the 70 S RNA. (For convenience, the largest RNA complex (50-70 S) isolated from any of the retroviruses studied was referred to as 70 S RNA.) These tRNA can be distinguished from the most tightly bound tRNA by the fact that they can be dissociated at lower temperatures. They occur in the same relative abundance as the tightly bound tRNA.