Phloridzin improves lipoprotein lipase activity in stress-loaded mice via AMPK phosphorylation
Phloridzin improves lipoprotein lipase activity in stress-loaded mice via AMPK phosphorylation
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DOI：
10.3109/09637486.2014.925429
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发表时间：
2014-10
影响因子：
3.9
通讯作者：
Guo-En Wang;Yi-Fang Li;Yan-ping Wu;Bun Tsoi;Shi-Jie Zhang;Ling-Fang Cao;H. Kurihara;Rong-Rong He-Rong
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文献类型：
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作者：
Guo-En Wang;Yi-Fang Li;Yan-ping Wu;Bun Tsoi;Shi-Jie Zhang;Ling-Fang Cao;H. Kurihara;Rong-Rong He-Rong
摘要长期的应激暴露会导致机体内稳态紊乱，引起多种生活方式疾病。根皮苷具有多种生物活性，但其对应激相关脂质代谢紊乱的影响尚不清楚。我们的研究结果表明，根皮苷改善血浆脂蛋白脂酶（LPL）活性和甘油三酯代谢在限制小鼠。根皮苷处理后，血管生成素样蛋白4（ANGPTL4）mRNA表达减少，AMP激活蛋白激酶（AMPK）磷酸化增加。抑制AMPK磷酸化后，根皮苷改善血浆LPL活性和抑制ANGPTL 4表达的作用被阻断。此外，心脏AMPK磷酸化，血浆LPL活性和ANGPTL4表达也受到根皮苷的影响，即使糖皮质激素受体被阻断。根皮苷可能通过直接激活AMPK途径下调ANGPTL4的表达。这一发现可为含根皮苷的食品和饮料在日常生活中的应用提供生化和营养学依据。
Abstract Long-term stress exposure can lead to disturbed homeostasis and cause many life-style diseases. Phloridzin possesses various bioactivities, but the understanding of the effects of phloridzin on stress-related lipid metabolism disorder is limited. Our results demonstrate that phloridzin improved plasma lipoprotein lipase (LPL) activity and triglyceride metabolism in restrained mice. A decrease of angiopoietin-like protein 4 (ANGPTL4) mRNA expression and an increase of AMP-activated protein kinase (AMPK) phosphorylation were observed after phloridzin treatment. After inhibiting AMPK phosphorylation, the effects of phloridzin on the amelioration of plasma LPL activity and suppression of ANGPTL4 expression were blocked. In addition, cardiac AMPK phosphorylation, plasma LPL activity and ANGPTL4 expression were also affected by phloridzin, even if the glucocorticoid receptor was blocked. Taken together, the down-regulation of ANGPTL4 expression by phloridzin was probably via a direct activation of AMPK pathway. This discovery can provide a biochemical and nutritional basis for the use of phloridzin-containing food and beverage in daily life.