Direct laser scribing of AgNPs@RGO biochip as a reusable SERS sensor for DNA detection
Direct laser scribing of AgNPs@RGO biochip as a reusable SERS sensor for DNA detection
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DOI：
10.1016/j.snb.2018.05.043
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发表时间：
2018-10-01
影响因子：
8.4
通讯作者：
Sun, Hong-Bo
中科院分区：
文献类型：
--
作者：
Han, Bing;Zhang, Yong-Lai;Sun, Hong-Bo
表面增强拉曼光谱(SEA)技术与微流控技术的结合，使得通过芯片上无标记DNA检测来诊断遗传病成为可能。然而，包括SERS衬底与微流控器件的集成、靶分子的可控捕获和释放等悬而未决的问题仍然具有挑战性。在这里，我们展示了一种简单的激光划片方法来制备基于银纳米粒子(AgNPs)和氧化石墨烯(GO)的生物芯片，作为可重复使用的用于DNA检测的SERS传感器。AgNPs@GO复合膜的可编程激光划片通过将复合材料剥离成分层的多孔结构，实现了由石墨烯支撑的AgNPs组成的敏感SERS通道的直接图案化。将SERS活性模式与微流控芯片相结合，形成了一种允许SERS检测DNA的生物芯片。DNA和石墨烯之间的非共价相互作用介导了DNA序列的可控捕获和释放，从而实现了高效的芯片上SERS检测和生物芯片的再生。SERS活性生物芯片的简单、绿色和高性价比的制造展示了其在生物分子传感和基因工程应用方面的巨大潜力。
The combination of surface-enhanced Raman spectroscopy (SEAS) technology with microfluidics makes it possible to diagnose genetic disease through label-free on-chip DNA detection. However, open problems including the integration of SERS substrate with microfluidic devices, controllable trapping and releasing of target molecules are still challenging. Here we demonstrate a facile laser scribing method to fabricate silver nano particles (AgNPs) and graphene oxide (GO) based biochips as a reusable SERS sensor for DNA detection. Programmable laser scribing of the AgNPs@GO composite film enables direct patterning of sensitive SERS channels that consist of graphene supported AgNPs by exfoliating the composites into hierarchical porous structures. Integrating the SERS-active patterns with a microfluidic chip forms a biochip for allowing SERS detection of DNA. The noncovalent interactions between DNA and graphene mediated controllable trapping and releasing of DNA sequences, enabling efficient on-chip SERS detection and the regeneration of the biochip. The simple, green and cost-effective fabrication of the SERS-active biochips reveals great potential for biomolecular sensing and genetic engineering applications.