Membrane marker and cell separation studies in Ph1-positive leukemia.
Membrane marker and cell separation studies in Ph1-positive leukemia.
复制标题
Ph1 阳性白血病的膜标记和细胞分离研究。
作者：
G. Janossy;R. Woodruff;A. Paxton;M. Greaves;D. Capellaro;B. Kirk;E. Innes;O. Eden;C. Lewis;D. Catovsky;A. Hoffbrand
从32例Ph ′-阳性白血病患者（其细胞与抗非I、非B急性淋巴细胞白血病（ALL）细胞的特异性抗血清反应）中，对5例典型患者进行了详细研究。其中3例患有Ph '阳性慢性粒细胞白血病（CML），后来发展为急性原始细胞危象伴淋巴细胞受累。另外两名患者表现为急性白血病，骨髓和淋巴母细胞混合。在所有5例病例中，淋巴母细胞表达与Ph '阴性非I、非B ALL相同的表型：它们与抗ALL和抗p28，33（抗la）血清反应，但缺乏胸腺细胞、B淋巴细胞和髓样细胞的分化标志物。在这些细胞群中检测到高水平的末端转移酶活性。在荧光激活细胞分选仪上将与抗ALL血清反应的原始细胞（ALL+）与非反应细胞（ALL-）分离。ALL细胞具有ALL母细胞的形态，而ALL细胞似乎是髓样的。这些研究也提示但不能证明ALL（淋巴样）和髓样人群都携带Ph'染色体。这些发现的含义是，至少有一些Ph '阳性白血病可能产生于前淋巴，前髓祖细胞和在原始细胞危机的一个可变比例的原始细胞可能保留其基本上干细胞样的特征。由于淋巴系统受累的患者有时对ALL的治疗有反应，因此早期诊断在临床上很重要。ALL抗原和末端转移酶的测定在淋巴母细胞危象的诊断中具有重要作用，特别是当淋巴母细胞与髓样细胞共存时。
Blood, Vol. 51, No. 5 (May), 1978 861 From 32 patients with Ph’-positive leukemia whose cells reacted with a specific antiserum made against non-I, non-B acute lymphoid leukemia (ALL) cells, five typical patients were studied in detail. Three of these had had Ph’-positive chronic myeloid leukemia (CML) and later developed acute blast crisis with lymphoid involvement. The other two patients presented with acute leukemia with a mixture of myeloid and lymphoid blasts. In all five cases lymphoid blasts expressed the same phenotype as Ph’-negative non-I, non-B ALL: they reacted with anti-ALL and anti-p28,33 (anti-la) sera but lacked differentiation markers of thymocytes, B lymphocytes, and myeloid cells. In these cell populations high levels of terminal transferase enzyme activity were detected. Blast cells reacting with anti-ALL serum (ALL+) were separated from unreactive cells (ALL-) on a fluorescence-activated cell sorter. ALL cells had the morphology of ALL blasts, while ALL cells appeared to be myeloid. These studies also suggest but do not prove that both ALL (lymphoid) and myeloid populations carried the Ph’ chromosome. The implication of these findings is that at least some Ph’-positive leukernias may arise from a prelymphoid, premyeloid progenitor and during blast crisis a variable proportion of blasts may retain their essentially stem cell-like characteristics. Since patients with lymphoid involvement sometimes respond to therapy designed for ALL, the early diagnosis is clinically important. Assays for ALL antigen and for terminal transferase have an important role in the diagnosis of lymphoid blast crisis, particularly if the lymphoblasts are present in the company of myelaid cells.