Transforming growth factor-β stimulates interleukin-11 production by human periodontal ligament and gingival fibroblasts
Transforming growth factor-β stimulates interleukin-11 production by human periodontal ligament and gingival fibroblasts
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DOI：
10.1111/j.1600-051x.2006.00898.x
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发表时间：
2006-03-01
影响因子：
6.7
通讯作者：
Ishikawa, I
中科院分区：
文献类型：
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作者：
Yashiro, R;Nagasawa, T;Ishikawa, I
背景：转化生长因子β（transforminggrowthfactor，TGF-β）是一种多功能多肽，广泛存在于骨基质中.白细胞介素（IL）-11是一种多效性细胞因子，对多种细胞类型具有作用。本研究旨在探讨TGF-β对人牙周膜细胞（PDL）和人牙龈成纤维细胞（HGF）分泌IL-11的调节作用。PDL和HGF用TGF-β刺激，有或没有蛋白激酶C（PKC）抑制剂和激活剂。通过实时聚合酶链反应（PCR）定量IL-11、骨形态发生蛋白-2（BMP-2）和TGF-β mRNA表达。结果：PDL和HGF在细胞表面均表达TGF-β受体I和TGF-β受体II。IL-11 mRNA的表达和IL-11的生产增加TGF-β在PDL和HGF，PDL中的值更高。PKC抑制剂部分抑制TGF-β诱导的PDL和HGF产生IL-11，而PKC激活剂则增强TGF-β诱导的PDL产生IL-11，TGF-β可上调PDL中TGF-β mRNA和BMP-2 mRNA的表达。
Background: Transforming growth factor (TGF)-beta is a potent multifunctional polypeptide, abundant in the bone matrix. Interleukin (IL)-11 is a pleiotropic cytokine with effects on multiple cell types. The present study was performed to evaluate the regulatory effects of TGF-beta on IL-11 production by human periodontal ligament cells (PDL) and human gingival fibroblasts (HGF).Materials and Methods: The expression of TGF-beta receptor in PDL and HGF were observed using flow cytometry. PDL and HGF were stimulated with TGF-beta with or without protein kinase C (PKC) inhibitors and activator. IL-11, bone morphogenetic protein-2 (BMP-2) and TGF-beta mRNA expression was quantified by real-time polymerase chain reaction (PCR). IL-11 production was measured using enzyme-linked immunosorbent assay.Results: PDL and HGF expressed both TGF-beta receptor I and TGF-beta receptor II on the cell surfaces. IL-11 mRNA expression and IL-11 production were augmented by TGF-beta in both PDL and HGF, with higher values in PDL. PKC inhibitors partially suppressed TGF-beta-induced IL-11 production in PDL and HGF, whereas activator enhanced it. TGF-beta mRNA and BMP-2 mRNA expression were up-regulated by TGF-beta in PDL.Conclusion: These results suggest that PDL produce IL-11 in response to TGF-beta.