In Vitro Study on Contribution of Oxidative Metabolism of Isolated Rabbit Heart Mitochondria to Myocardial Reperfusion Injury
In Vitro Study on Contribution of Oxidative Metabolism of Isolated Rabbit Heart Mitochondria to Myocardial Reperfusion Injury
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离体兔心脏线粒体氧化代谢对心肌再灌注损伤影响的体外研究
DOI：
10.1161/01.res.55.2.168
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发表时间：
1984
影响因子：
20.1
通讯作者：
T. Kagawa
中科院分区：
文献类型：
--
作者：
H. Otani;Hidehiko Tanaka;Takeo Inoue;M. Umemoto;K. Omoto;Kazuho Tanaka;Tadashi Sato;T. Osako;A. Masuda;A. Nonoyama;T. Kagawa
The present study was performed to clarify oxygen-induced damage following myocardial reperfusion, using three mitochondrial preparations from isolated rabbit hearts (non-ischemic hearts and those subjected to 40 and 90 minutes of normothermic global ischemia). The viability of mitochondria was evaluated by adenosine triphosphate generation. The extent of mitochondrial injury produced by reactive oxygen metabolism was assessed by the intensity of hydroxyl radical signal detected with electron spin resonance spectroscopy and the reduction of coenzyme Q10 level. The greatest oxygen-induced injury was observed in 40-minute ischemic mitochondria exposed to pure oxygen. The use of superoxide dismutase and catalase satisfactorily prevented the oxygen-induced injury. Moreover, the net adenosine triphosphate generation of the 40-minute ischemic mitochondria was comparable to that of the nonischemic mitochondria without the enzymes. These results suggest that reperfusion of the ischemic myocardium with viable mitochondria is deleterious, because mitochondria are susceptible to injury resulting from oxidative metabolism, and that the use of superoxide dismutase, together with catalase, is beneficial for the restoration of cardiac function after ischemia.
