Carbohydrate-coated lanthanide-doped upconverting nanoparticles for lectin recognition
Carbohydrate-coated lanthanide-doped upconverting nanoparticles for lectin recognition
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DOI：
10.1039/c0jm01617a
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发表时间：
2010-01-01
影响因子：
--
通讯作者：
Capobianco, John A.
中科院分区：
文献类型：
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作者：
Bogdan, Nicoleta;Vetrone, Fiorenzo;Capobianco, John A.
在这项研究中，我们开发了上转换镧系元素（Ln（3+））掺杂的纳米粒子与糖树枝状聚合物能够识别凝集素的基础上发光共振能量转移（LRET）。将聚酰胺-胺（PAMAM）树枝状大分子通过直接配体交换吸附在NaGdF 4：Er 3+，Yb 3+上转换纳米粒子（LnNPs）表面，然后在胺表面与对异硫氰酸苯基α-D-甘露糖苷之间形成有效的硫脲键，用于共价糖衍生化。所得的水分散性和生物相容性的甘露糖包被的PAMAM-LnNP用于识别凝集素，伴刀豆球蛋白A（甘露糖结合蛋白）与四甲基罗丹明（RITC-Con A）通过LRET从上转换甘露糖包被的PAMAM-LnNP缀合，所述上转换甘露糖包被的PAMAM-LnNP充当能量供体，所述RITC标记的凝集素分子充当能量受体。随着凝集素浓度的增加，观察到在980 nm激发后从甘露糖包被的PAMAM-LnNP到RITC-Con A的能量转移现象，其中在585 nm处的发射增加。这些新的碳水化合物包被的LnNP被证明是一种新的工具，以监测与凝集素在水溶液中的样品的结合相互作用。
In this study we have developed upconverting lanthanide (Ln(3+))-doped nanoparticles conjugated with glycodendrimers capable of recognizing lectins based on luminescence resonance energy transfer (LRET). Poly(amidoamine) (PAMAM) dendrimers were adsorbed on the surface of NaGdF4:Er3+, Yb3+ upconverting nanoparticles (LnNPs) via direct ligand-exchange followed by efficient thiourea linkage formation between the amine surface and p-isothiocyanatophenyl alpha-D-mannopyranoside for covalent carbohydrate derivatization. The resulting water dispersible and biocompatible mannose-coated PAMAM-LnNPs were used to recognize a lectin, Concanavalin A (a mannose binding protein) conjugated with tetramethylrhodamine (RITC-Con A) via LRET from the upconverting mannose-coated PAMAM-LnNPs, which act as energy donors to the RITC-labeled lectin molecules serving as energy acceptors. The energy transfer phenomenon from the mannose-coated PAMAM-LnNPs, following 980 nm excitation, to the RITC-Con A was observed with increasing emissions at 585 nm, as the concentration of lectin increases. These novel carbohydrate-coated LnNPs prove to be a novel tool to monitor the binding interaction with lectins in aqueous samples.