Extensive skinning of cell membrane diminishes the force-inhibiting effect of okadaic acid on smooth muscles of Guinea pig hepatic portal vein.
Extensive skinning of cell membrane diminishes the force-inhibiting effect of okadaic acid on smooth muscles of Guinea pig hepatic portal vein.
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DOI：
10.2170/jjphysiol.52.141
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发表时间：
2002-04
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影响因子：
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通讯作者：
Masaru Watanabe;H. Takano‐Ohmuro
中科院分区：
文献类型：
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作者：
Masaru Watanabe;H. Takano‐Ohmuro
在豚鼠肝门静脉研究了细胞膜透化（结皮）对冈田酸抑制血管平滑肌收缩的影响。在不存在Ca（2+）的情况下，用1 μ M冈田酸预处理抑制了用金黄色葡萄球菌α毒素或β-七叶皂苷透化的制剂中随后的次最大Ca（2+）诱导的收缩，但在用皂苷或Triton X-100处理的制剂中没有。来自制剂的洗脱液的SDS-PAGE表明，在用皂苷或Triton X-100去皮的制剂中，冈田酸的抑制作用的丧失是由于具有67至200 kDa的分子量的一些细胞组分的泄漏。
The influences of cell membrane permeabilization (skinning) on the okadaic acid-induced inhibition of vascular smooth muscle contraction were studied in guinea pig hepatic portal vein. Pretreatment by 1 microM okadaic acid in the absence of Ca(2+) suppressed subsequent submaximal Ca(2+)-induced contraction in preparations permeabilized with Staphylococcus aureus alpha-toxin or beta-escin, but not in those treated with saponin or Triton X-100. The SDS-PAGE of elutants from the preparation suggests that the loss of the inhibitory effect of okadaic acid in preparations skinned with saponin or Triton X-100 results from the leakage of some cellular components with a molecular mass of 67 to 200 kDa.