Efficient Genetic Transformation of Lotus corniculatus L. and Growth of Transformed Plants in Field
Efficient Genetic Transformation of Lotus corniculatus L. and Growth of Transformed Plants in Field
复制标题
百脉根高效遗传转化及转化植株的田间生长
DOI：
10.1023/a:1027357620951
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发表时间：
2003
影响因子：
1.5
通讯作者：
M. Neskovic
中科院分区：
文献类型：
--
作者：
R. Nikolic;N. Mitić;S. Ninković;J. Miljuš;M. Neskovic
以新莲花的根段为材料，建立了一套高效的不定芽再生和遗传转化方案。博科品种。用发根农杆菌A4M70GUS接种生根农杆菌A4M70GUS，在含有0.2mgdm−-3-苄基氨基嘌呤的培养基上再生不定芽。经生根驯化后，在试验田种植转化植株。将它们的形态特征与对照组进行比较。没有出现致病基因表型的迹象。转化子株高显著高于对照，叶面积差异不显著。在T0转化植株和T1代幼苗中证明了葡萄糖醛酸酶活性和FuidA基因的存在。得出的结论是。生根可能是将理想的基因转移到鸟脚三叶基因组的一种选择载体。
An efficient protocol for shoot regeneration and genetic transformation was applied to root segments of a newLotus corniculatusL. cultivar Bokor. The shoots, that regenerated on root segments, were inoculated withAgrobacterium rhizogenesA4M70GUS, and produced hairy roots, which on media with 0.2 mg dm−3benzylaminopurine, regenerated shoots. After rooting and acclimation, the transformed plants were planted in the experimental field. Their morphological traits were compared to controls. No signs of therolgenes phenotype were present. The transformants were significantly taller than controls, while there were no significant differences in the leaf area. The glucuronidase activity and the presence ofuidAgene was demonstrated in transformed plants of T0and in seedlings of T1generations. It is concluded thatA. rhizogenescould be a vector of choice for the transfer of desirable genes into the bird's foot trefoil genome.