Nucleotide sequences and the molecular evolution of the DMA and DMB genes of the bovine major histocompatibility complex.
Nucleotide sequences and the molecular evolution of the DMA and DMB genes of the bovine major histocompatibility complex.
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牛主要组织相容性复合体的 DMA 和 DMB 基因的核苷酸序列和分子进化。
DOI：
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发表时间：
1995
期刊：
影响因子：
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通讯作者：
Y. Aida
中科院分区：
文献类型：
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作者：
M. Niimi;Y. Nakai;Y. Aida
分离并表征了编码牛主要组织相容性复合物 (MHC) II 类 DM α 和 β 链的 cDNA 克隆。 BoLA-DMA cDNA克隆MA7编码260个氨基酸的初级翻译产物，其中包括26个氨基酸的信号肽和234个氨基酸的成熟多肽。 BoLA-DMB cDNA 克隆 MB6 编码 262 个氨基酸的初级翻译产物，具有 18 个氨基酸的信号肽和 244 个氨基酸的成熟多肽。序列比较和系统发育树的构建表明，这两个克隆与人类和小鼠 DM 基因的关系比与传统牛 II 类 α 链和 β 链基因的关系更密切。因此，由于牛 DMA 和 DMB 基因与其他 II 类序列非常不同，并且在牛、小鼠和人类同源物中显示出很强的保守性 (> 70%)，因此这些 cDNA 克隆中的每一个似乎都编码一种功能产物，该产物可能执行重要的功能，正如先前在小鼠和人类研究中所确定的那样。
cDNA clones encoding the bovine major histocompatibility complex (MHC) class II DM alpha- and beta-chains were isolated and characterized. The BoLA-DMA cDNA clone, MA7, encoded a primary translated product of 260 amino acids, which included a signal peptide of 26 amino acids and a mature polypeptide of 234 amino acids. The BoLA-DMB cDNA clone, MB6, encoded a primary translated product of 262 amino acids, with a signal peptide of 18 amino acids and a mature polypeptide of 244 amino acids. Comparison of the sequences and construction of a phylogenetic tree revealed that both clones are more closely related to human and mouse DM genes than to genes for conventional bovine class II alpha- and beta-chains. Thus, since the bovine DMA and DMB genes are so different from other class II sequences and show evidence of strong conservation (> 70%) among the bovine, mouse and human homologues, it seems likely that each of these cDNA clones encodes a functional product, which might perform an important function, as previously established in studies in mouse and man.